Document Detail

Molecular orientation behavior of silk sericin film as revealed by ATR infrared spectroscopy.
MedLine Citation:
PMID:  16004444     Owner:  NLM     Status:  MEDLINE    
This paper reports the structure-dependent molecular orientation behavior of sericin, an adhesive silk protein secreted by silkworm, Bombyx mori. Although application of sericin as a biomaterial is anticipated because of its unique characteristics, sericin's physicochemical properties remain unclear, mainly because of its vulnerability to heat or alkaline treatment during separation from fibroin threads. This study employed intact sericin obtained from fibroin-deficient mutant silkworm to investigate the relationship between molecular orientation and the secondary structure of sericin. Sericin films were artificially stretched after moistening with aqueous ethanol of various concentrations. The resulting molecular orientation was analyzed using polarized infrared spectroscopy. These analyses indicated that formation of aggregated strands among extended sericin chains induced by ethanol treatment is the key to generating molecular orientation. Strong intermolecular hydrogen bonds are inferred to allow aggregated strands' stretching-force transmission, thereby causing molecular orientation.
Hidetoshi Teramoto; Mitsuhiro Miyazawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomacromolecules     Volume:  6     ISSN:  1525-7797     ISO Abbreviation:  Biomacromolecules     Publication Date:    2005 Jul-Aug
Date Detail:
Created Date:  2005-07-11     Completed Date:  2005-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2049-57     Citation Subset:  IM    
New Silk Materials Laboratory, Insect Biotechnology and Sericology Department, National Institute of Agrobiological Sciences, 1-4-8 Gohda, Okaya, Nagano 394-0021, Japan.
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MeSH Terms
Electrophoresis, Polyacrylamide Gel
Protein Structure, Secondary
Sericins / chemistry*
Spectroscopy, Fourier Transform Infrared / methods*
Reg. No./Substance:

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