Document Detail


Molecular motions in HIV-1 gp120 mutants reveal their preferences for different conformations.
MedLine Citation:
PMID:  17227719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both the crystal structures of the HIV-1 gp120 core bound by the CD4 and antigen 17b, and the SIV gp120 core pre-bound by CD4 are known. We built the homology models of gp120 with loops V3 and V4 in the CD4-complex, CD4-free and CD4-unliganded states, and models of the 375 S/W and 423 I/P mutants in the CD4-free and unliganded states, respectively. CONCOORD was utilized for generating ensembles of the seven gp120 models that were analyzed by essential dynamics analyses to identify their preferred concerted motions. The revealed large-scale concerted motions are related to either the receptor association/release or the conformational transition between different conformational states. Essential subspace overlap analyses were performed to quantitatively distinguish the preference for conformational transitions between states of the gp120 mutants and further to ascertain what kind of conformational state that the mutants prefer to adopt. Results indicate that the 375 S/W mutant, in which the tryptophan indole group is predicted to occupy the phe43 pocket in the gp120 interior, favors a conformation close to the CD4-bound state. However, the other mutant 423 I/P inclines to prevent the formation of bridging sheet and stabilize the conformation in the unliganded state. Our theoretical analyses are in agreement with experimentally determined mutation effects, and can be extended to a new approach to design or screen mutants that have effects on conformation/function of a protein.
Authors:
Shu-Qun Liu; Ci-Qun Liu; Yun-Xin Fu
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-15
Journal Detail:
Title:  Journal of molecular graphics & modelling     Volume:  26     ISSN:  1093-3263     ISO Abbreviation:  J. Mol. Graph. Model.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-02     Completed Date:  2007-10-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9716237     Medline TA:  J Mol Graph Model     Country:  United States    
Other Details:
Languages:  eng     Pagination:  306-18     Citation Subset:  IM    
Affiliation:
Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, PR China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acid Substitution
Antigens, CD4 / metabolism
Computer Simulation
HIV Envelope Protein gp120 / chemistry*,  genetics*,  metabolism
Humans
Models, Molecular
Molecular Sequence Data
Motion
Multiprotein Complexes
Point Mutation
Protein Binding
Protein Conformation
Sequence Homology, Amino Acid
Thermodynamics
Chemical
Reg. No./Substance:
0/Antigens, CD4; 0/HIV Envelope Protein gp120; 0/Multiprotein Complexes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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