Document Detail

Molecular methods in the diagnostic microbiology laboratory: when to start and where to stop?
MedLine Citation:
PMID:  10769616     Owner:  NLM     Status:  MEDLINE    
The introduction of molecular diagnostic methods in the microbiologic diagnostic laboratory is subject to a number of practical and financial constraints requiring the elaboration of a sound strategy. Validation of the tests may require the use of an expanded gold standard or the application of novel statistical methods such as latent class analysis. The procedures should be constantly evaluated for false positive an false negative results. The introduction of molecular methods will not only depend on their performance for each individual microorganism, but also on the clinical relevance of the diagnostic question asked, the prevalence of the clinical problem and whether the new methods are added to the procedures in use or will replace them. Therefore no general rules can be proposed, strategies have to be elaborated for each infectious agent or clinical syndrome. Strategies concerning the use of molecular diagnostic techniques for the diagnosis of Mycobacterium tuberculosis, Chlamydia trachomatis, meningo-encephalitis syndrome and respiratory infections, are discussed. Their role in typing of microorganisms and the detection of previously unknown infectious agents are mentioned.
M Ieven
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Verhandelingen - Koninklijke Academie voor Geneeskunde van België     Volume:  62     ISSN:  0302-6469     ISO Abbreviation:  Verh. K. Acad. Geneeskd. Belg.     Publication Date:  2000  
Date Detail:
Created Date:  2000-05-03     Completed Date:  2000-05-03     Revised Date:  2005-11-16    
Medline Journal Info:
Nlm Unique ID:  0413210     Medline TA:  Verh K Acad Geneeskd Belg     Country:  BELGIUM    
Other Details:
Languages:  eng     Pagination:  15-29; discussion 29-30     Citation Subset:  IM    
Laboratory for Microbiology, University Hospital Antwerp, Edegem.
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MeSH Terms
Diagnostic Techniques and Procedures* / trends
Laboratories* / trends
Microbiological Techniques* / trends

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