| Molecular mechanisms of isocyanate induced oncogenic transformation in ovarian epithelial cells. | |
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MedLine Citation:
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PMID: 20685613 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Ovarian surface epithelium is under constant physiological pressure to maintain its integrity. Environmental toxic exposure can contribute to degenerative pathologies including ovarian cancer. Based on our current understanding, we aimed at listing mechanistic insights that contribute to ovarian carcinogenesis after exposure to methyl isocyanate, an ubiquitous environmental pollutant. Ovarian epithelial cells manifested a persistent DNA damage response along with increased accumulation of GADD45, p21, p16(INK4A) and pRb proteins upon treatment. Increase in cell size and β-gal positive staining showing inception of premature senescence with morphological transformation and structural and numerical chromosomal abnormalities were also observed. Immuno-FISH analysis illustrated early loss of TRF2 protein suggestive of telomeric dysfunction due to premature senescence and plausible association with chromosomal and microsatellite instability. Soft-agar assay displayed neoplasticity in treated cells demonstrating onset of malignant transformation. These results indicate that isocyanate exposure alters ovarian epithelial cell proliferation and might lead to ovarian dysfunction and carcinogenesis. |
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Authors:
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Gorantla Venkata Raghuram; Neelam Pathak; Deepika Jain; Hariom Panwar; Hemant Pandey; Subodh Kumar Jain; Pradyumna Kumar Mishra |
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Publication Detail:
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Type: Journal Article Date: 2010-06-08 |
Journal Detail:
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Title: Reproductive toxicology (Elmsford, N.Y.) Volume: 30 ISSN: 1873-1708 ISO Abbreviation: Reprod. Toxicol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-09-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8803591 Medline TA: Reprod Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 377-86 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Bhopal Memorial Hospital & Research Centre, Bhopal, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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