| Molecular mechanisms in C-Phycocyanin induced apoptosis in human chronic myeloid leukemia cell line-K562. | |
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MedLine Citation:
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PMID: 15242812 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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C-Phycocyanin (C-PC), the major light harvesting biliprotein from Spirulina platensis is of greater importance because of its various biological and pharmacological properties. It is a water soluble, non-toxic fluorescent protein pigment with potent anti-oxidant, anti-inflammatory and anti-cancer properties. In the present study the effect of highly purified C-PC was tested on growth and multiplication of human chronic myeloid leukemia cell line (K562). The results indicate significant decrease (49%) in the proliferation of K562 cells treated with 50 microM C-PC up to 48 h. Further studies involving fluorescence and electron microscope revealed characteristic apoptotic features like cell shrinkage, membrane blebbing and nuclear condensation. Agarose electrophoresis of genomic DNA of cells treated with C-PC showed fragmentation pattern typical for apoptotic cells. Flow cytometric analysis of cells treated with 25 and 50 microM C-PC for 48 h showed 14.11 and 20.93% cells in sub-G0/G1 phase, respectively. C-PC treatment of K562 cells also resulted in release of cytochrome c into the cytosol and poly(ADP) ribose polymerase (PARP) cleavage. These studies also showed down regulation of anti-apoptotic Bcl-2 but without any changes in pro-apoptotic Bax and thereby tilting the Bcl-2/Bax ratio towards apoptosis. These effects of C-PC appear to be mediated through entry of C-PC into the cytosol by an unknown mechanism. The present study thus demonstrates that C-PC induces apoptosis in K562 cells by cytochrome c release from mitochondria into the cytosol, PARP cleavage and down regulation of Bcl-2. |
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Authors:
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Jagu Subhashini; Suraneni V K Mahipal; Madhava C Reddy; Metukuri Mallikarjuna Reddy; Aparna Rachamallu; Pallu Reddanna |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical pharmacology Volume: 68 ISSN: 0006-2952 ISO Abbreviation: Biochem. Pharmacol. Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-07-09 Completed Date: 2004-08-30 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 453-62 Citation Subset: IM |
Affiliation:
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Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Apoptosis* Cell Division / drug effects Cytochromes c / metabolism DNA Fragmentation / drug effects Humans Immunoblotting K562 Cells Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology Phycocyanin / pharmacology* Poly(ADP-ribose) Polymerases / metabolism Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-bcl-2 / metabolism bcl-2-Associated X Protein |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/BAX protein, human; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 11016-15-2/Phycocyanin; 9007-43-6/Cytochromes c; EC 2.4.2.30/Poly(ADP-ribose) Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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