| Molecular mechanisms of band 3 inhibitors. 3. Translocation inhibitors. | |
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MedLine Citation:
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PMID: 3801448 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During the translocation of the band 3 transport site between the inward- and outward-facing orientations, the Cl- transport site complex passes through a transition state lying on the reaction pathway between the two extreme orientations. Niflumic acid, 2-[(7-nitrobenzofurazan-4-yl)amino]ethanesulfonate, and 2,4,6-trichlorobenzenesulfonate each are translocation blockers that can bind to both the inward- and outward-facing conformations of band 3. The principal mechanism of these inhibitors is a reduction in the translocation rate, since they have essentially no effect on the apparent KD for Cl- binding to the transport site and the migration of Cl- between the transport site and solution. Instead, these inhibitors raise the free energy of formation of the transition state during translocation and thereby can lock the transport site into either the inward- or outward-facing orientation. In contrast, 2,4-dinitrofluorobenzene (DNFB) appears to restrict the accessibility of the transport site to solution Cl-; also, the DNFB reaction rate is increased by Cl-, suggesting that DNFB modification may occur during translocation. Thus DNFB is proposed to trap the Cl--transport site complex site during translocation to yield a conformation intermediate to the inward- and outward-facing orientations. A model is presented for the molecular mechanism of transport across biological membranes. The transport machinery is proposed to contain greater than or equal to 6 transmembrane helices that surround a central channel containing a sliding hydrophobic barrier. The transport site lies between two of the channel-forming helices and remains stationary while the hydrophobic barrier slides from one end of the channel to the other, thereby exposing the transport site to the opposite solution compartment. |
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Authors:
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J J Falke; S I Chan |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 25 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1986 Dec |
Date Detail:
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Created Date: 1987-03-20 Completed Date: 1987-03-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7899-906 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anion Exchange Protein 1, Erythrocyte
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antagonists & inhibitors* Benzenesulfonates / pharmacology* Biological Transport, Active / drug effects Chlorides / blood* Dinitrofluorobenzene / pharmacology Erythrocyte Membrane / drug effects, metabolism Humans Kinetics Models, Biological Nicotinic Acids / pharmacology* Niflumic Acid / pharmacology* Oxadiazoles / pharmacology* Taurine / analogs & derivatives*, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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GM 22432/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anion Exchange Protein 1, Erythrocyte; 0/Benzenesulfonates; 0/Chlorides; 0/NBD-taurine; 0/Nicotinic Acids; 0/Oxadiazoles; 104778-51-0/2,4,6-trichlorobenzenesulfonate; 107-35-7/Taurine; 4394-00-7/Niflumic Acid; 70-34-8/Dinitrofluorobenzene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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