| Molecular mechanism of pancreatic β-cell adaptive proliferation: studies during pregnancy in rats and in vitro. | |
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MedLine Citation:
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PMID: 21069579 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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There is a widespread interest in defining factors and mechanisms that stimulate proliferation of pancreatic islet β-cells. Pregnancy is a special periods when the pancreatic islet display a highly reproducible physiological proliferation. However, the molecular mechanism of β-cell proliferation during pregnancy is unclear. Here, we used cDNA expression array to explore gene expression profiles of islet at various stages of pregnancy in rats. Differentially expressed genes related to islet proliferation were screened by bioinformatics methods, and further verified by real-time PCR, RT-PCR, and Western blotting. Compared with control group, expressions of hundreds of genes were changed during pregnancy. The differentially expressed genes related to islet proliferation were mainly distributed in three groups: genes involved in transcription regulator activity, genes involved in apoptosis or tumor, and genes for Wnt signaling pathway. Among these genes, expressions of Nupr1, Atf3, Btg2, β-catenin, and c-Myc mRNA were up-regulated during pregnancy. A prominent expression of Nupr1 and Atf3 protein was observed in islets on day 10.5 of pregnancy, i.e., with earlier time phases than proliferation peak. Moreover, we found that prolactin (PRL) can increase the proliferation of β-cell in vitro, which is accompanied by up-regulation of Atf3 and Nupr1, indicating that they may play a crucial role in PRL-induced pancreatic β-cell growth. In conclusion, our results suggest that the transcription factor Nupr1, Atf3, and Wnt pathway may play an important role in adaptive proliferation of pancreatic islets during pregnancy in rats. |
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Authors:
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Guofang Chen; Cuiping Liu; Ying Xue; Xiaodong Mao; Kuanfeng Xu; Chao Liu |
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Publication Detail:
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Type: Journal Article Date: 2010-11-11 |
Journal Detail:
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Title: Endocrine Volume: 39 ISSN: 1559-0100 ISO Abbreviation: Endocrine Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9434444 Medline TA: Endocrine Country: United States |
Other Details:
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Languages: eng Pagination: 118-27 Citation Subset: IM |
Affiliation:
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Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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