| Molecular mechanism of matrine-induced apoptosis in leukemia K562 cells. | |
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MedLine Citation:
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PMID: 17163597 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Matrine, a low toxic alkaloid purified from the Chinese herb Kushen, has been reported to induce apoptosis in leukemia K562 cells. In this study, the mechanism underling this apoptotic event was investigated. Treatment of K562 cells with matrine resulted in inhibition of cell survival more significantly than treatment of non-cancer fibroblast NIH3T3 cells. When K562 cells were incubated with matrine in higher than 0.2 mg/ml doses for 48 hours, the apoptotic cells were increased and both poly (ADP-ribose) polymerase (PARP) and caspase-3 were cleaved in a dose dependent manner. General caspase inhibitor (z-VAD-fmk) or caspase-3 inhibitor (z-DEVD-fmk) almost completely suppressed matrine-induced apoptosis. In addition, matrine increased proapoptotic protein bax and caused the release of cytochrome C. Taken together, the results suggest that matrine induces a cytochrome C-mediated, caspase-dependent apoptosis. |
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Authors:
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Xiao-Shan Liu; Jikai Jiang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of Chinese medicine Volume: 34 ISSN: 0192-415X ISO Abbreviation: Am. J. Chin. Med. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-12-13 Completed Date: 2007-04-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7901431 Medline TA: Am J Chin Med Country: Singapore |
Other Details:
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Languages: eng Pagination: 1095-103 Citation Subset: IM |
Affiliation:
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Center for Molecular Biology, School of Medicine, Shantou University, Shantou, Guangdong, 515031, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaloids
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pharmacology* Amino Acid Chloromethyl Ketones / pharmacology Antineoplastic Agents, Phytogenic / pharmacology* Apoptosis / drug effects* Caspase 3 / antagonists & inhibitors, metabolism Cysteine Proteinase Inhibitors / pharmacology Cytochromes c / metabolism Dose-Response Relationship, Drug Humans K562 Cells Oligopeptides / pharmacology Poly(ADP-ribose) Polymerases / metabolism Quinolizines / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Alkaloids; 0/Amino Acid Chloromethyl Ketones; 0/Antineoplastic Agents, Phytogenic; 0/Cysteine Proteinase Inhibitors; 0/Oligopeptides; 0/Quinolizines; 0/benzoylcarbonyl-aspartyl-glutamyl-valyl-aspartyl-fluoromethyl ketone; 0/benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; 519-02-8/matrine; 9007-43-6/Cytochromes c; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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