| Molecular mechanism of damage and repair of mouse thymus lymphocytes induced by radiation. | |
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MedLine Citation:
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PMID: 12150745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the role of apoptosis in radiation-induced mouse thymus lymphocyte damage and repair and provide the basis for understanding the molecular mechanism of radiation-induced lymphocyte damage and repair as well as the prevention and treatment of acute radiation sickness. METHODS: We studied the dynamic changes of apoptosis of mouse thymus lymphocytes and the expression of bax and bcl-2 gene products after 2, 4, 6 and 8 Gy of whole body gamma-irradiation using in situ terminal labeling, DNA electrophoresis and immunohistochemical techniques. RESULTS: At the early stage after irradiation, the percentage of apoptotic lymphocytes increased rapidly in accordance with the increasing of radiation doses, while the counts of the thymus and peripheral lymphocytes decreased sharply, showing an opposite change to lymphocyte apoptosis. After 6 Gy gamma-irradiation, typical morphological characteristics of thymus apoptotic lymphocytes in early, middle and late stages were found by transmission electron microscopy. The thymus lymphocytes displayed characteristic DNA ladders 4 hr and 8 hr after 2-6 Gy gamma-irradiation,using DNA gel electrophoresis techniques. Abnormal expression of bcl-2 and bax gene products were shown in irradiated lymphocytes. CONCLUSIONS: Apoptosis plays an important role in the process of radiation-induced mouse thymus lymphocyte damage and repair. Bcl-2 and Bax proteins may regulate the process of lymphocyte apoptosis. |
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Authors:
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Yufang Cui; Hong Yang; Shuxia Wu; Linlu Gao; Yabing Gao; Ruiyun Peng; Xuemei Cui; Chengqi Xiong; Wenhua Hu; Dewen Wang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chinese medical journal Volume: 115 ISSN: 0366-6999 ISO Abbreviation: Chin. Med. J. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-08-01 Completed Date: 2002-09-10 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7513795 Medline TA: Chin Med J (Engl) Country: China |
Other Details:
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Languages: eng Pagination: 1070-3 Citation Subset: IM |
Affiliation:
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Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China. gaoyb@nic.bmi.ac.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / radiation effects* Dose-Response Relationship, Radiation Gamma Rays Lymphocytes / physiology, radiation effects* Male Mice Proto-Oncogene Proteins / analysis Proto-Oncogene Proteins c-bcl-2 / analysis Thymus Gland / pathology, radiation effects Time Factors bcl-2-Associated X Protein |
| Chemical | |
Reg. No./Substance:
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0/Bax protein, mouse; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein |
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