Document Detail


Molecular live cell bioimaging in stem cell research.
MedLine Citation:
PMID:  22901252     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Functional heterogeneity within stem and progenitor cells has been shown to influence cell fate decisions. Similarly, intracellular signaling activated by external stimuli is highly heterogeneous and its spatiotemporal activity is linked to future cell behavior. To quantify these heterogeneous states and link them to future cell fates, it is important to observe cell populations continuously with single cell resolution. Live cell imaging in combination with fluorescent biosensors for signaling activity serves as a powerful tool to study cellular and molecular heterogeneity and the long-term biological effects of signaling. Here, we describe these methodologies, their advantages over classical approaches, and we illustrate how they could be applied to improve our understanding of the importance of heterogeneous cellular and molecular responses to external signaling cues.
Authors:
Max Endele; Timm Schroeder
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1266     ISSN:  1749-6632     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18-27     Citation Subset:  IM    
Copyright Information:
© 2012 New York Academy of Sciences.
Affiliation:
Research Unit Stem Cell Dynamics, Helmholtz Center Munich-German Research Center for Environmental Health, Neuherberg, Germany.
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