Document Detail


Molecular interactions between the specialist herbivore Manduca sexta (Lepidoptera, Sphigidae) and its natural host Nicotiana attenuata. VIII. An unbiased GCxGC-ToFMS analysis of the plant's elicited volatile emissions.
MedLine Citation:
PMID:  19136568     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Treating wounds in Nicotiana attenuata leaves with Manduca sexta oral secretions (W+OS) mimics most changes elicited by M. sexta herbivory, but an unbiased analysis of the effect of the different OS constituents on volatile emissions is lacking. We used two-dimensional gas chromatography/time-of-flight (GCxGC-ToF) mass spectrometry combined with multivariate statistics to parse volatiles into regulatory patterns. Volatiles released by wounding alone and by the alkalinity of OS were assessed by applying a buffer known to mimic the pH-mediated changes of OS elicitation (pectin methyl esterase activation and methanol release). The activities of fatty acid amino acid conjugates, well-known elicitors of antiherbivore defenses, and of 2-hydroxyoctadecatrienoic acid, a newly discovered signal in OS, were determined. Approximately 400 analytes were detected after deconvolution and alignment of GCxGC data; 35 volatiles were significantly regulated upon W+OS. Two-thirds of these were specifically regulated by OS, being either amplified (most terpenoids and certain hexenylesters) or strongly repressed (many short-chain alcohols and some aromatic and hexenylester derivatives). Fatty acid amino acid conjugates played a central role in this pattern of regulation, since they induced the emission of half of OS-elicited volatiles and inhibited the production of almost all OS-repressed volatiles; 2-hydroxyoctadecatrienoic acid influenced emission of trans-alpha-bergamotene, while other unknown OS constituents amplified hexenylester production. We conclude that the complex bouquet of herbivory-elicited volatiles results from the complex modulations of the wound response by diverse cues found in OS. This work also underscores the value of ultra-high-resolution GCxGC-ToF analysis combined with the nontargeted mining of the resulting data.
Authors:
Emmanuel Gaquerel; Alexander Weinhold; Ian T Baldwin
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Publication Detail:
Type:  Journal Article     Date:  2009-01-09
Journal Detail:
Title:  Plant physiology     Volume:  149     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-04     Completed Date:  2009-05-14     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1408-23     Citation Subset:  IM    
Affiliation:
Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, Jena 07745, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Gas
Cluster Analysis
Fatty Acids / analysis,  chemistry
Feeding Behavior*
Manduca / physiology*
Mass Spectrometry*
Principal Component Analysis
Tobacco / parasitology*
Volatile Organic Compounds / analysis*,  chemistry
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Volatile Organic Compounds
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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