| Molecular insights into eukaryotic chemotaxis. | |
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MedLine Citation:
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PMID: 1743439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Many cells display directed migration toward specific compounds. The best-studied eukaryotic models of chemotaxis are polymorphonuclear leukocytes, which respond to formylated peptides and Dictyostelium amoebas, which respond to extracellular cAMP. In both cell types, chemoattractants bind to surface receptors that contain seven transmembrane domains and interact with G proteins. Some cells, such as fibroblasts, undergo chemotaxis toward compounds whose receptors lack this motif and transmit their signals by other mechanisms. The cytosolic changes elicited by chemoattractants include increased levels of cAMP, cGMP, inositol phosphates, and calcium. These changes are correlated with actin polymerization and other cytoskeletal events that result in preferential extension of pseudopods toward the chemoattractant. Dictyostelium cell lines in which specific genes have been disrupted have demonstrated the necessity of a cAMP receptor (cAR1) and a G protein alpha-subunit (G alpha 2) for responsiveness to cAMP. Other proteins, such as myosin heavy chain and several actin binding proteins, are dispensible although their absence does affect the details of chemotaxis. The disruption of other relevant genes and the genetic reconstitution of chemotaxis in cells lacking crucial proteins should reveal many clues about this complicated and fascinating process. |
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Authors:
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M J Caterina; P N Devreotes |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S.; Review |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 5 ISSN: 0892-6638 ISO Abbreviation: FASEB J. Publication Date: 1991 Dec |
Date Detail:
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Created Date: 1992-01-16 Completed Date: 1992-01-16 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3078-85 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chemotaxis / physiology* Chemotaxis, Leukocyte / physiology Dictyostelium / metabolism* Eukaryotic Cells / metabolism* GTP-Binding Proteins / metabolism Models, Biological Receptors, Cyclic AMP / metabolism Second Messenger Systems / physiology |
| Grant Support | |
ID/Acronym/Agency:
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GM28007/GM/NIGMS NIH HHS; GM39933/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Cyclic AMP; EC 3.6.1.-/GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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