Document Detail


Molecular and immunochemical characteristics of monoclonal and recombinant antibodies specific to bisphenol A.
MedLine Citation:
PMID:  12843665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four anti-bisphenol A monoclonal antibodies (mabs) were obtained and each characterized by an enzyme-linked immunosorbent assay (ELISA). Among these mabs, BBA-2187 was the most reactive towards bisphenol A. The quantitation limit of the ELISA assay for bisphenol A was 0.13 ng/ml, which is more sensitive than the other immunoassays reported. Then, the cDNA clones encoding variable heavy and variable light chains of these four mabs were isolated, and used for construction of four single-chain Fv (scFv) antibody genes, which were expressed in Escherichia coli cells. The reactivity of four scFv antibodies towards bisphenol A in ELISA was comparable to those of the parent mabs. The most sensitive assay was achieved with BBA-2187scFv. Its cross-reactivity to the related compounds was similar to that of the parent mab. Based on the reactivity of heterologous combinations of VH and VL fragments, it was found that the unique structure of the framework region 2 in the VL of BBA-2187 appeared to be important for specific assembly together with the VH.
Authors:
Kosuke Nishi; Mikio Takai; Kosuke Morimune; Hideo Ohkawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  67     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-04     Completed Date:  2004-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1358-67     Citation Subset:  IM    
Affiliation:
Research Center for Environmental Genomics, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal / biosynthesis,  genetics*,  immunology*
Antibody Affinity
Cloning, Molecular*
Cross Reactions
DNA, Complementary / genetics
Enzyme-Linked Immunosorbent Assay / standards
Escherichia coli / genetics
Female
Immunoglobulin Fragments / immunology
Mice
Mice, Inbred BALB C
Phenols / analysis,  immunology*
Recombinant Proteins / genetics,  immunology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/DNA, Complementary; 0/Immunoglobulin Fragments; 0/Phenols; 0/Recombinant Proteins; 80-05-7/bisphenol A

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