Document Detail


Molecular imaging of human embryonic stem cells: keeping an eye on differentiation, tumorigenicity and immunogenicity.
MedLine Citation:
PMID:  17172859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human embryonic stem cells (hESCs) are capable of differentiation into every cell type of the human being. They are under extensive investigation for their regenerative potential in a variety of debilitating diseases. However, the field of hESC research is still in its infancy, as there are several critical issues that need to be resolved before clinical translation. Two major concerns are the ability of undifferentiated hESCs to form teratomas and the possibility of a provoked immune reaction after transplantation of hESCs into a new host. Therefore, it is imperative to develop noninvasive imaging modalities that allow for longitudinal, repetitive, and quantitative assessment of transplanted cell survival, proliferation, and migration in vivo. Reporter gene-based molecular imaging offers these characteristics and has great potential in the field of stem cell therapy. Moreover, it has recently been shown that reporter gene imaging can be combined with therapeutic strategies. Here, we provide an outline of the current status of hESC research and discuss the concerns of tumorigenicity and immunogenicity. Furthermore, we describe how molecular imaging can be utilized to follow and resolve these issues.
Authors:
Koen E A van der Bogt; Rutger-Jan Swijnenburg; Feng Cao; Joseph C Wu
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Publication Detail:
Type:  Journal Article     Date:  2006-12-01
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  5     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-25     Completed Date:  2007-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2748-52     Citation Subset:  IM    
Affiliation:
Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, California, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation*
Cell Lineage
Ectoderm / cytology
Embryonic Stem Cells / cytology*,  immunology*
Endoderm / cytology
Humans
Luminescent Proteins / metabolism
Mesoderm / cytology
Stem Cell Transplantation
Teratoma / pathology*
Chemical
Reg. No./Substance:
0/Luminescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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