| Molecular imaging of fibrin in a breast cancer xenograft mouse model. | |
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MedLine Citation:
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PMID: 22960948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RATIONALE AND OBJECTIVES: Fibrin deposition has been indicated within the stroma of a majority of solid tumors. Here we assess the feasibility of using the established fibrin-specific probe EP-2104R for noninvasive imaging of fibrin in the context of breast cancer. METHODS: EP-2104R, untargeted gadopentetate dimeglumine (Gd-DTPA), and a newly synthesized nonfibrin binding control linear peptide (CLP) were compared using steady-state and dynamic contrast-enhanced magnetic resonance imaging in a breast cancer xenograft mouse model at 9.4 T. RESULTS: EP-2104R transiently enhanced both tumor core and tumor periphery, but only the enhancement in the tumor periphery persisted even 90 minutes after EP-2104R administration. However, untargeted Gd-DTPA and CLP are not retained in the tumor periphery. The half-life of EP-2104R in the tumor periphery (103 ± 18 minutes) is significantly longer (P < 0.05) than that of either Gd-DTPA (29.6 ± 2.4 minutes) or CLP (42.4 ± 1.5 minutes), but the rate of clearance is similar for all the 3 probes from the tumor core. The presence of high concentrations of fibrin in the tumor periphery was corroborated using immunohistochemistry with a fibrin-specific antibody. CONCLUSIONS: The persistent enhancement observed in the tumor periphery with EP-2104R is likely a result of its fibrin-specific binding rather than its size and demonstrates the feasibility of EP-2104R for molecular imaging of fibrin in tumor stroma. |
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Authors:
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Ritika Uppal; Zdravka Medarova; Christian T Farrar; Guangping Dai; Anna Moore; Peter Caravan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Investigative radiology Volume: 47 ISSN: 1536-0210 ISO Abbreviation: Invest Radiol Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-10 Completed Date: 2013-02-06 Revised Date: 2013-05-15 |
Medline Journal Info:
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Nlm Unique ID: 0045377 Medline TA: Invest Radiol Country: United States |
Other Details:
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Languages: eng Pagination: 553-8 Citation Subset: IM |
Affiliation:
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Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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diagnosis,
pathology Animals Breast Neoplasms / diagnosis*, pathology Disease Models, Animal Feasibility Studies Female Fibrin* Gadolinium / diagnostic use* Gadolinium DTPA / diagnostic use* Mice Molecular Imaging* Peptides / diagnostic use* Transplantation, Heterologous |
| Grant Support | |
ID/Acronym/Agency:
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P41RR14075/RR/NCRR NIH HHS; R01 EB009062/EB/NIBIB NIH HHS; R01 HL109448/HL/NHLBI NIH HHS; R01EB009062/EB/NIBIB NIH HHS; R01HL109448/HL/NHLBI NIH HHS; S10RR025563/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/EP-2104R; 0/Peptides; 7440-54-2/Gadolinium; 80529-93-7/Gadolinium DTPA; 9001-31-4/Fibrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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