| Molecular imaging of angiogenic therapy in peripheral vascular disease with alphanubeta3-integrin-targeted nanoparticles. | |
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MedLine Citation:
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PMID: 20665780 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Noninvasive molecular imaging of angiogenesis could play a critical role in the clinical management of peripheral vascular disease patients. The alpha(nu)beta(3)-integrin, a well-established biomarker of neovascular proliferation, is an ideal target for molecular imaging of angiogenesis. This study investigates whether MR molecular imaging with alpha(nu)beta(3)-integrin-targeted perfluorocarbon nanoparticles can detect the neovascular response to angiogenic therapy. Hypercholesterolemic rabbits underwent femoral artery ligation followed by no treatment or angiogenic therapy with dietary L-arginine. MR molecular imaging performed 10 days after vessel ligation revealed increased signal enhancement in L-arginine-treated animals compared to controls. Furthermore, specifically targeted nanoparticles produced two times higher MRI signal enhancement compared to nontargeted particles, demonstrating improved identification of angiogenic vasculature with biomarker targeting. X-ray angiography performed 40 days postligation revealed that L-arginine treatment increased the development of collateral vessels. Histologic staining of muscle capillaries revealed a denser pattern of microvasculature in L-arginine-treated animals, confirming the MR and X-ray imaging results. The clinical application of noninvasive molecular imaging of angiogenesis could lead to earlier and more accurate detection of therapeutic response in peripheral vascular disease patients, enabling individualized optimization for a variety of treatment strategies. |
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Authors:
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Patrick M Winter; Shelton D Caruthers; John S Allen; Kejia Cai; Todd A Williams; Gregory M Lanza; Samuel A Wickline |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 64 ISSN: 1522-2594 ISO Abbreviation: Magn Reson Med Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-28 Completed Date: 2011-01-04 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: United States |
Other Details:
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Languages: eng Pagination: 369-76 Citation Subset: IM |
Affiliation:
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Washington University, St. Louis, Missouri, USA. patrick@cmrl.wustl.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Angiogenesis Inhibitors / administration & dosage* Animals Arginine / administration & dosage* Drug Delivery Systems / methods Integrin alphaVbeta3 / analysis* Magnetic Resonance Imaging / methods* Molecular Probe Techniques* Nanoparticles / diagnostic use Peripheral Arterial Disease / diagnosis*, drug therapy* Prognosis Rabbits Treatment Outcome |
| Grant Support | |
ID/Acronym/Agency:
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R01 EB001704-01/EB/NIBIB NIH HHS; R01 EB001704-03/EB/NIBIB NIH HHS; R01 EB01704/EB/NIBIB NIH HHS; R01 HL073646/HL/NHLBI NIH HHS; R01 HL073646-02/HL/NHLBI NIH HHS; R01 HL073646-07/HL/NHLBI NIH HHS; U54 CA119342/CA/NCI NIH HHS; U54 CA119342-01/CA/NCI NIH HHS; U54 CA119342-05/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Angiogenesis Inhibitors; 0/Integrin alphaVbeta3; 74-79-3/Arginine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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