Document Detail

Molecular imaging of VEGF receptors in angiogenic vasculature with single-chain VEGF-based probes.
MedLine Citation:
PMID:  17351626     Owner:  NLM     Status:  MEDLINE    
We describe a new generation of protein-targeted contrast agents for multimodal imaging of the cell-surface receptors for vascular endothelial growth factor (VEGF). These receptors have a key role in angiogenesis and are important targets for drug development. Our probes are based on a single-chain recombinant VEGF expressed with a cysteine-containing tag that allows site-specific labeling with contrast agents for near-infrared fluorescence imaging, single-photon emission computed tomography or positron emission tomography. These probes retain VEGF activities in vitro and undergo selective and highly specific focal uptake into the vasculature of tumors and surrounding host tissue in vivo. The fluorescence contrast agent shows long-term persistence and co-localizes with endothelial cell markers, indicating that internalization is mediated by the receptors. We expect that multimodal imaging of VEGF receptors with these probes will be useful for clinical diagnosis and therapeutic monitoring, and will help to accelerate the development of new angiogenesis-directed drugs and treatments.
Marina V Backer; Zoya Levashova; Vimalkumar Patel; Brian T Jehning; Kevin Claffey; Francis G Blankenberg; Joseph M Backer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-03-11
Journal Detail:
Title:  Nature medicine     Volume:  13     ISSN:  1078-8956     ISO Abbreviation:  Nat. Med.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-06     Completed Date:  2007-11-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9502015     Medline TA:  Nat Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  504-9     Citation Subset:  IM    
SibTech, Inc., 705 North Mountain Road, Newington, Connecticut 06111, USA.
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MeSH Terms
Cell Line, Tumor
Contrast Media*
Diagnostic Imaging*
Mice, Inbred BALB C
Mice, SCID
Microscopy, Confocal
Microscopy, Fluorescence / methods
Neovascularization, Physiologic / physiology*
Positron-Emission Tomography / methods
Receptors, Vascular Endothelial Growth Factor / metabolism*
Recombinant Proteins / metabolism*
Tomography, Emission-Computed, Single-Photon / methods
Grant Support
1 P50 CA114747/CA/NCI NIH HHS; CA064436/CA/NCI NIH HHS; R21 EB001946/EB/NIBIB NIH HHS; R43 CA113080/CA/NCI NIH HHS
Reg. No./Substance:
0/Contrast Media; 0/Recombinant Proteins; EC, Vascular Endothelial Growth Factor

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