Document Detail


Molecular identity of uncoupling proteins in thermogenic skunk cabbage.
MedLine Citation:
PMID:  18974196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thermogenic skunk cabbage has been reported to have two types of uncoupling protein (UCP), a typical 6-transmembrane (TM) SrUCPA and an atypical 5-TM SrUCPB. To verify further the role of SrUCPs in thermogenic skunk cabbage, we examined the molecular identity of SrUCPs in more detail. Both mRNA and genomic analyses supported the presence of SrUCPA, but not SrUCPB. Furthermore, SrUCP protein purified from spadix mitochondria was identified as SrUCPA by mass spectrometry. These results clearly indicate that SrUCPA is the major expressed UCP in skunk cabbage, and the presence of atypical SrUCPB is unlikely to be associated with thermogenesis of skunk cabbage.
Authors:
Yasuko Ito-Inaba; Yamato Hida; Hitoshi Mori; Takehito Inaba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-30
Journal Detail:
Title:  Plant & cell physiology     Volume:  49     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-08     Completed Date:  2009-01-06     Revised Date:  2009-12-23    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1911-6     Citation Subset:  IM    
Affiliation:
Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, Morioka, 020-8550 Japan. ykoito@iwate-u.ac.jp
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB459551
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Araceae / genetics*,  metabolism
Gene Expression Regulation, Plant
Genome, Plant
Ion Channels / genetics*,  metabolism
Mitochondria / genetics,  metabolism
Mitochondrial Proteins / genetics*,  metabolism
Molecular Sequence Data
Plant Proteins / genetics*,  metabolism
RNA Splicing
RNA, Messenger / genetics
RNA, Plant / genetics
Temperature
Chemical
Reg. No./Substance:
0/Ion Channels; 0/Mitochondrial Proteins; 0/Plant Proteins; 0/RNA, Messenger; 0/RNA, Plant; 0/mitochondrial uncoupling protein

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