Document Detail

Molecular identification of coliform bacteria from colicky breastfed infants.
MedLine Citation:
PMID:  19604166     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine the presence of intestinal coliform bacteria in colicky vs healthy infants. STUDY DESIGN: We isolated coliform strains from faeces and performed quantitative bacterial cultures in 41 colicky and 39 healthy breastfed infants, identified using PCR with species-specific primers, strain-specific Automated Ribotyping and the API-50E kit for Enterobacteriaceae to identify the most frequent strains. RESULTS: Coliform strains were more abundant in colicky infants (median 6.04 log(10) CFU/g faeces, range 2.00-8.76) vs controls (median 4.47 log(10) CFU/g faeces, range 1.00-8.08) (p = 0.026). Escherichia coli, Klebsiella pneumoniae, K. oxytoca, Enterobacter cloacae, E. aerogenes and Enterococcus faecalis were the predominant species in colicky and healthy infants. The counts of each bacterial species differed between the two groups, and the difference was significant (p = 0.002) for E. coli: median 6.30 log(10) CFU/g faeces (range 3.00-8.74) in colicky infants, and median 4.70 log(10) CFU/g faeces (range 2.00-5.85) in controls. CONCLUSIONS: This is the first study to evaluate the colonization patterns of gas-forming coliforms in colicky infants and healthy controls identified by molecular methods. Coliform bacteria, particularly Escherichia coli, were found to be more abundant in colicky infants. Our data could help to shed light on the cause of infantile colic.
F Savino; L Cordisco; V Tarasco; R Calabrese; E Palumeri; D Matteuzzi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-09
Journal Detail:
Title:  Acta paediatrica (Oslo, Norway : 1992)     Volume:  98     ISSN:  1651-2227     ISO Abbreviation:  Acta Paediatr.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-21     Completed Date:  2009-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205968     Medline TA:  Acta Paediatr     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  1582-8     Citation Subset:  IM    
Department of Pediatrics, Regina Margherita Children Hospital, University of Turin, Torino, Italy.
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MeSH Terms
Breast Feeding*
Colic / etiology,  microbiology*
Colony Count, Microbial
Cross-Sectional Studies
Enterobacteriaceae / chemistry,  genetics,  isolation & purification*
Escherichia coli / isolation & purification
Feces / microbiology
Infant, Newborn
Intestines / microbiology*
Polymerase Chain Reaction
Sequence Analysis, DNA
Statistics, Nonparametric

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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