Document Detail


Molecular identification and characterization of clustered regularly interspaced short palindromic repeat (CRISPR) gene cluster in Taylorella equigenitalis.
MedLine Citation:
PMID:  23275249     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Clustered regularly interspaced short palindromic repeats (CRISPRs), of approximately 10,000 base pairs (bp) in length, were shown to occur in the Japanese Taylorella equigenitalis strain, EQ59. The locus was composed of the putative CRISPRs-associated with 5 (cas5), RAMP csd1, csd2, recB, cas1, a leader region, 13 CRISPR consensus sequence repeats (each 32 bp; 5'-TCAGCCACGTTCGCGTGGCTGTGTGTTTAAAG-3'). These were in turn separated by 12 non repetitive unique spacer regions of similar length. In addition, a leader region, a transposase/IS protein, a leader region, and cas3 were also seen. All seven putative open reading frames carry their ribosome binding sites. Promoter consensus sequences at the -35 and -10 regions and putative intrinsic ρ-independent transcription terminator regions also occurred. A possible long overlap of 170 bp in length occurred between the recB and cas1 loci. Positive reverse transcription PCR signals of cas5, RAMP csd1, csd2-recB/cas1, and cas3 were generated. A putative secondary structure of the CRISPR consensus repeats was constructed. Following this, CRISPR results of the T. equigenitalis EQ59 isolate were subsequently compared with those from the Taylorella asinigenitalis MCE3 isolate.
Authors:
Yasushi Hara; Kyohei Hayashi; Takuya Nakajima; Shizuko Kagawa; Akihiro Tazumi; John E Moore; Motoo Matsuda
Related Documents :
15134879 - Vex-capture: a new technique that allows in vivo excision, cloning, and broad-host-rang...
17921289 - Evolution and functional characterization of the rh50 gene from the ammonia-oxidizing b...
9600949 - Genomic evidence for two functionally distinct gene classes.
15723179 - Genetic discovery in xylella fastidiosa through sequence analysis of selected randomly ...
20421399 - Isecp1-mediated transposition and homologous recombination can explain the context of b...
7045589 - Plasmid cole1 conjugal mobility: the nature of bom, a region required in cis for transfer.
7682529 - A method for the isolation of rna from streptococcus salivarius and its application to ...
1685479 - Structure and sequence of the human homeobox gene hox7.
16478449 - Design of shewanella-specific 16s rrna primers and application to analysis of shewanell...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-30
Journal Detail:
Title:  Folia microbiologica     Volume:  -     ISSN:  1874-9356     ISO Abbreviation:  Folia Microbiol. (Praha)     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376757     Medline TA:  Folia Microbiol (Praha)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71, Chuo-ku, Sagamihara, 252-5201, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Microembolism and Catheter Ablation II: Effects of Cerebral Microemboli Injection in a Canine Model.
Next Document:  Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goa...