Document Detail


Molecular heterogeneity of developing retinal ganglion and amacrine cells revealed through single cell gene expression profiling.
MedLine Citation:
PMID:  17444492     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During development of the central nervous system (CNS), cycling uncommitted progenitor cells give rise to a variety of distinct neuronal and glial cell types. As these different cell types are born they progress from newly specified cells to fully differentiated neurons and glia. In order to define the developmental processes of individual cell types, single cell expression profiling was carried out on developing ganglion and amacrine cells of the murine retina. Individual cells from multiple developmental stages were isolated and profiled on Affymetrix oligonucleotide arrays. Two-color fluorescent in situ hybridization on dissociated retinas was used to verify and extend the microarray results by allowing quantitative measurements of a large number of cells coexpressing two genes. Together, these experiments have yielded an expanded view of the processes underway in developing retinal ganglion and amacrine cells, as well as several hundred new marker genes for these cell types. In addition, this study has allowed for the definition of some of the molecular heterogeneity both between developing ganglion and amacrine cells and among subclasses of each cell type.
Authors:
Jeffrey M Trimarchi; Michael B Stadler; Botond Roska; Nathan Billings; Ben Sun; Brandon Bartch; Constance L Cepko
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  502     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-04-25     Completed Date:  2007-06-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1047-65     Citation Subset:  IM    
Copyright Information:
(c) 2007 Wiley-Liss, Inc.
Affiliation:
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
Amacrine Cells / cytology,  metabolism*
Animals
Cell Differentiation / genetics*
Cells, Cultured
Gene Expression Profiling / methods
Gene Expression Regulation, Developmental / genetics*
Genetic Markers / genetics
In Situ Hybridization, Fluorescence
Mice
Nerve Tissue Proteins / biosynthesis,  genetics
Neurofilament Proteins / biosynthesis,  genetics
Retina / cytology,  embryology*,  metabolism*
Retinal Ganglion Cells / cytology,  metabolism*
Retinal Rod Photoreceptor Cells / cytology,  metabolism
Stem Cells / cytology,  metabolism
Transcription Factor AP-2 / genetics
Transcription, Genetic / genetics
Grant Support
ID/Acronym/Agency:
EY08064/EY/NEI NIH HHS; F32 EY014495/EY/NEI NIH HHS; T32 EY007145/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Genetic Markers; 0/Nerve Tissue Proteins; 0/Neurofilament Proteins; 0/Tcfap2b protein, mouse; 0/Transcription Factor AP-2; 0/neurofilament protein L

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