| Molecular genotyping of HIV-1 in 61 patients with AIDS from Lomé, Togo. | |
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MedLine Citation:
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PMID: 9890418 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To study the distribution of HIV types and genotypes, in Lomé, Togo, a random population of patients who met the clinical criteria of the Bangui definition of AIDS and were positive with two independent screening assays for antibodies to HIV-1 group M, HIV-2, and HIV-1 group O was selected. HIV RNA from serum samples was reverse-transcribed and amplified with degenerate primers annealing to conserved regions of the HIV-1, HIV-2, and HIV-O gag gene. Amplicons were directly sequenced using an automated sequencer. A 262-271-bp (strain-dependent) fragment of the gag gene from each patient was phylogenetically analyzed and compared to the corresponding gag sequences of published HIV-1 sequences of known African genotypes. Genotype A was found in 48 of 60 patient amplicons (80%), subdivided into two clusters. Ten patients (16.7%) were HIV-1 genotype G; one was genotype D and one genotype H. HIV-1 genotype B was not found. Amplicons from two patients contained sequence ambiguities, requiring cloning and sequencing of the gag insert. One patient (T52) was apparently infected with HIV-1 genotypes A and G; whereas HIV-1 from patient T139 was of genotype A, with 2/10 clones having a three-codon insertion at nucleotide position 1142 of the gag gene. HIV-1 genotype A is dominant in Togo; genotype G is frequent and genotype B has not been found. |
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Authors:
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F J Davies; O d'Almeida; E Timmers; J d'Ameida; M Fasken; K Bassabi; H Lee; J P Allain |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of medical virology Volume: 57 ISSN: 0146-6615 ISO Abbreviation: J. Med. Virol. Publication Date: 1999 Jan |
Date Detail:
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Created Date: 1999-03-25 Completed Date: 1999-03-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 25-30 Citation Subset: IM; X |
Affiliation:
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Department of Haematology, University of Cambridge, England. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acquired Immunodeficiency Syndrome
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epidemiology,
virology* Adult Antibodies, Viral / blood Antibody Specificity Female Genes, gag / genetics Genotype HIV-1 / genetics*, immunology HIV-2 / immunology Humans Male Molecular Epidemiology Phylogeny Polymerase Chain Reaction RNA, Viral / blood, genetics Togo / epidemiology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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