Document Detail


Molecular genotyping of HIV-1 in 61 patients with AIDS from Lomé, Togo.
MedLine Citation:
PMID:  9890418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study the distribution of HIV types and genotypes, in Lomé, Togo, a random population of patients who met the clinical criteria of the Bangui definition of AIDS and were positive with two independent screening assays for antibodies to HIV-1 group M, HIV-2, and HIV-1 group O was selected. HIV RNA from serum samples was reverse-transcribed and amplified with degenerate primers annealing to conserved regions of the HIV-1, HIV-2, and HIV-O gag gene. Amplicons were directly sequenced using an automated sequencer. A 262-271-bp (strain-dependent) fragment of the gag gene from each patient was phylogenetically analyzed and compared to the corresponding gag sequences of published HIV-1 sequences of known African genotypes. Genotype A was found in 48 of 60 patient amplicons (80%), subdivided into two clusters. Ten patients (16.7%) were HIV-1 genotype G; one was genotype D and one genotype H. HIV-1 genotype B was not found. Amplicons from two patients contained sequence ambiguities, requiring cloning and sequencing of the gag insert. One patient (T52) was apparently infected with HIV-1 genotypes A and G; whereas HIV-1 from patient T139 was of genotype A, with 2/10 clones having a three-codon insertion at nucleotide position 1142 of the gag gene. HIV-1 genotype A is dominant in Togo; genotype G is frequent and genotype B has not been found.
Authors:
F J Davies; O d'Almeida; E Timmers; J d'Ameida; M Fasken; K Bassabi; H Lee; J P Allain
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical virology     Volume:  57     ISSN:  0146-6615     ISO Abbreviation:  J. Med. Virol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-25     Completed Date:  1999-03-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  25-30     Citation Subset:  IM; X    
Affiliation:
Department of Haematology, University of Cambridge, England.
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MeSH Terms
Descriptor/Qualifier:
Acquired Immunodeficiency Syndrome / epidemiology,  virology*
Adult
Antibodies, Viral / blood
Antibody Specificity
Female
Genes, gag / genetics
Genotype
HIV-1 / genetics*,  immunology
HIV-2 / immunology
Humans
Male
Molecular Epidemiology
Phylogeny
Polymerase Chain Reaction
RNA, Viral / blood,  genetics
Togo / epidemiology
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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