Document Detail


Molecular and genetic characterization of a non-metastatic human esophageal cancer cell line, T.Tn expressing non-functional mutated p53.
MedLine Citation:
PMID:  12168098     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lymph node metastasis is commonly found in esophageal squamous cell carcinoma (SCC). In this study, we examined the molecular and genetic characteristics of a human esophageal SCC cell line, T.Tn. T.Tn cells formed tumors at s.c. tissue in nude mice when inoculated with Matrigel, but did not metastasize to any organs. T.Tn cells expressed low level of proMMP2 and a trace level of proMMP9. However, T.Tn cells expressed high level of TIMP1 and TIMP2, and beta-catenin and E-cadherin. We found a point mutation of p53 gene at codon 213 (CAT-->CGT) in T.Tn cells. The mutated-p53 protein did not show transcriptional activity on p21(waf1), MDM2 and Bax promoters. Thus, T.Tn cells are low tumorigenic and weakly invasive but not metastasizing in nude mice, and T.Tn cells are suitable parental cells for establishing a model system to study invasion and metastasis of esophageal SCC.
Authors:
Taro Sakai; Tadashi Furihata; Hitoshi Kawamata; Fumie Omotehara; Yasuhiro Shinagawa; Johji Imura; Keiichi Kubota; Akira Terano; Takahiro Fujimori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  21     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-08-08     Completed Date:  2003-03-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  547-52     Citation Subset:  IM    
Affiliation:
Department of Surgical and Molecular Pathology, Dokkyo University School of Medicine, 880 Kitakobayashi, Mibu, Shimotsuga, Tochigi 321-0293, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cadherins / biosynthesis
Carcinoma, Squamous Cell / genetics*,  metabolism,  pathology*,  secondary
Cell Division / physiology
Collagenases / biosynthesis
Cytoskeletal Proteins / biosynthesis,  genetics
Enzyme Precursors / biosynthesis
Esophageal Neoplasms / genetics*,  metabolism,  pathology*
Gelatinases / biosynthesis
Humans
Immunohistochemistry
Matrix Metalloproteinase 9
Metalloendopeptidases / biosynthesis
Mice
Mice, Nude
Mutation
Neoplasm Invasiveness
Neoplasm Metastasis
Neoplasm Transplantation
Tissue Inhibitor of Metalloproteinase-1 / biosynthesis,  secretion
Tissue Inhibitor of Metalloproteinase-2 / biosynthesis,  secretion
Trans-Activators / biosynthesis,  genetics
Transcriptional Activation
Transplantation, Heterologous
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / biosynthesis,  genetics*,  physiology
beta Catenin
Chemical
Reg. No./Substance:
0/CTNNB1 protein, human; 0/Cadherins; 0/Catnb protein, mouse; 0/Cytoskeletal Proteins; 0/Enzyme Precursors; 0/Tissue Inhibitor of Metalloproteinase-1; 0/Trans-Activators; 0/Tumor Suppressor Protein p53; 0/beta Catenin; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC 3.4.24.-/Collagenases; EC 3.4.24.-/Gelatinases; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.-/pro-matrix metalloproteinase 9; EC 3.4.24.-/progelatinase; EC 3.4.24.35/Matrix Metalloproteinase 9

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