Document Detail


Molecular and functional characterization of the murine glucocerebrosidase gene.
MedLine Citation:
PMID:  1317175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A genomic clone of glucocerebrosidase (D-glucosyl-N-acyl-sphingosine glucohydrolase; E.C. 3.2.1.45) purified from a genomic library derived from a Balb/c mouse was analyzed by restriction mapping and nucleotide sequencing of its promoter and protein coding regions. Promoter activity was functionally assessed by ligation of a 2 kb glucocerebrosidase fragment to the protein coding segment of a bacterial neomycin resistance gene. Smaller segments of the 5' flanking sequence were then analyzed for their ability to initiate transcription of the chloramphenicol acetyltransferase reporter gene. A 319 bp Eco RI-Bgl II fragment (containing 259 bp upstream of the cDNA 5' limit) ligated to the chloramphenicol acetyltransferase open reading frame produced considerable activity.
Authors:
E D Carstea; G J Murray; R R O'Neill
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  184     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-06-23     Completed Date:  1992-06-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1477-83     Citation Subset:  IM    
Affiliation:
Developmental and Metabolic Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M89944;  M89949;  M97581;  M97582;  M97583;  M97584;  M97585;  M97586;  M97587;  M97588;  M97589
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Base Sequence
Chloramphenicol O-Acetyltransferase / genetics,  metabolism
Cloning, Molecular
Exons
Genes*
Genomic Library
Glucosylceramidase / genetics*,  metabolism
Humans
Kanamycin Kinase
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Open Reading Frames
Phosphotransferases / genetics,  metabolism
Promoter Regions, Genetic*
Recombinant Fusion Proteins
Restriction Mapping
Sequence Homology, Nucleic Acid
Transfection
Chemical
Reg. No./Substance:
0/Recombinant Fusion Proteins; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase; EC 2.7.-/Phosphotransferases; EC 2.7.1.95/Kanamycin Kinase; EC 3.2.1.45/Glucosylceramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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