| Molecular flexibility and discontinuous translocation of a non-templated polymerase. | |
| | |
MedLine Citation:
|
PMID: 15033355 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Little is known regarding the translocation of non-templated nucleic acid polymerases with respect to single-stranded primers. VP55, the vaccinia virus poly(A) polymerase, translocates as it processively adds a approximately 3-7 adenylate tail to primers possessing only three ribouridylate residues (as an (rU)(2)-N(15)-rU motif), and a approximately 25-30 adenylate tail to primers that are more U-rich. Here, three models were addressed for the translocation of VP55 with respect to its primer, namely: (a) rigid protein/rigid nucleic acid; (b) flexible protein/rigid nucleic acid; (c) rigid protein/flexible nucleic acid. Analysis of free and covalently VP55-attached primers favored either (b) or a version of (c) incorporating a passive steric block, and suggested two regions of relative motion between polymerase and primer. Inclusion of a 6nt uridylate-rich patch at the primer 3' end switched the polymerase from approximately 3-7 nt to approximately 25-30 nt tail addition without affecting initial binding affinity. By synthesizing this patch as a (rU/dC) pool, discontinuous polymerase movements could be detected. |
| | |
Authors:
|
L Johnson; S Liu; P D Gershon |
Related Documents
:
|
11295195 - Rapid detection, identification, and enumeration of escherichia coli by fluorescence in... 11125075 - The stanford microarray database. 11086725 - Synthesis and properties of chiral peptide nucleic acids with a n-aminoethyl-d-proline ... 3271435 - Conformational studies of nucleic acids: iii. empirical multiple correlation functions ... 8932305 - A bifunctional urease enhances survival of pathogenic yersinia enterocolitica and morga... 9772735 - Determination of diethanolamine and n-nitrosodiethanolamine in fatty acid diethanolamides. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of molecular biology Volume: 337 ISSN: 0022-2836 ISO Abbreviation: J. Mol. Biol. Publication Date: 2004 Apr |
Date Detail:
|
Created Date: 2004-03-22 Completed Date: 2004-04-28 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985088R Medline TA: J Mol Biol Country: England |
Other Details:
|
Languages: eng Pagination: 843-56 Citation Subset: IM |
Affiliation:
|
Department of Medical Biochemistry and Genetics, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, Houston, TX 77030, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Polynucleotide Adenylyltransferase
/
metabolism* Protein Binding Protein Transport RNA / biosynthesis* Vaccinia virus / enzymology*, genetics |
| Grant Support | |
ID/Acronym/Agency:
|
GM51953/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
63231-63-0/RNA; EC 2.7.7.19/Polynucleotide Adenylyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: phi29 DNA polymerase-terminal protein interaction. Involvement of residues specifically conserved am...
Next Document: The biotin repressor: modulation of allostery by corepressor analogs.