Document Detail


Molecular features of phospholipids that affect glycolipid transfer protein-mediated galactosylceramide transfer between vesicles.
MedLine Citation:
PMID:  16777057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The glycolipid transfer protein (GLTP)-mediated movement of galactosylceramide from model membrane donor vesicles to acceptor vesicles is sensitive to the membrane environment surrounding the glycolipid. GLTP can catalyze the transfer of a fluorescently labeled GSL, anthrylvinyl-galactosylceramide (AV-GalCer), from vesicles composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and dipalmitoylphosphatidylcholine matrices, but not from vesicles prepared from N-palmitoylsphingomyelin, regardless of the cholesterol content of the vesicles. In this study, we have examined the structural features of sphingomyelin (SM) that are responsible for its inhibition of the rate of GLTP-catalyzed transfer of AV-GalCer. The rate of glycolipid transfer was enhanced when the N-palmitoyl chain of SM was replaced with an N-oleoyl chain. Analogs of N-palmitoyl-SM in which the 4,5-double bond of the long-chain base is reduced or the 3-hydroxy group is removed did not inhibit GLTP-catalyzed transfer of AV-GalCer. When the donor vesicles were prepared with phosphatidylcholines or ether-linked phosphatidylcholine analogs, the transfer rates of AV-GalCer increased with increasing degree of unsaturation. The rate of AV-GalCer transfer was strongly dependent on the unsaturation degree of the acyl and/or alkyl chains. For ester-linked PCs, the transfer rate increased in the order DPPC<POPC<DOPC, which have 0, 1, and 2 cis double bonds, respectively.
Authors:
Matts Nylund; Matti A Kjellberg; Julian G Molotkovsky; Hoe-Sup Byun; Robert Bittman; Peter Mattjus
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-05-07
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1758     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-07-25     Completed Date:  2006-09-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  807-12     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.
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MeSH Terms
Descriptor/Qualifier:
Carrier Proteins / metabolism*
Cholesterol / metabolism
Galactosylceramides / metabolism*
Phospholipids / metabolism
Sphingomyelins / metabolism
Grant Support
ID/Acronym/Agency:
HL-083187/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Galactosylceramides; 0/Phospholipids; 0/Sphingomyelins; 0/lipid transfer protein; 57-88-5/Cholesterol

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