| Molecular evolution of the vesicle coat component betaCOP in Toxoplasma gondii. | |
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MedLine Citation:
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PMID: 17485226 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Coatomer coated (COPI) vesicles play a pivotal role for multiple membrane trafficking steps throughout the eukaryotic cell. Our focus is on betaCOP, one of the most well known components of the COPI multi-protein complex. Amino acid differences in betaCOP may dictate functional divergence across species during the course of evolution, especially with regards to the evolutionary pressures on obligate intracellular parasites. A bioinformatic analysis of betaCOP amino acid sequences was conducted for 49 eukaryotic species. Cloning and sequence analysis of the Toxoplasma gondii betaCOP homologue revealed several amino acid insertions unique to T. gondii and one C-terminal insertion that is unique to apicomplexan parasites. These findings led us to investigate the possibility that betaCOP experienced functional divergence during the course of its evolution. Bayesian phylogenetic analysis revealed a tree consistent with pan eukaryote distribution and long-branch lengths were observed among the apicomplexans. Further analysis revealed that kinetoplast betaCOP underwent the most amount of change, leading to perhaps an overall change of function. In comparison, T. gondii exhibited subtle yet specific amino acid changes. The amino acid substitutions did not occur in the same places as other lineages, suggesting that TgbetaCOP has a role specific to the apicomplexans. Our work identifies 48 residues that are likely to be functionally important when comparing apicomplexan, kinetoplastid, and fungal betaCOP. |
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Authors:
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Sherri S Smith; Stacy L Pfluger; Elizabeth Hjort; Andrew G McArthur; Kristin M Hager |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-03-24 |
Journal Detail:
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Title: Molecular phylogenetics and evolution Volume: 44 ISSN: 1055-7903 ISO Abbreviation: Mol. Phylogenet. Evol. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-08-22 Completed Date: 2007-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9304400 Medline TA: Mol Phylogenet Evol Country: United States |
Other Details:
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Languages: eng Pagination: 1284-94 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556-0369, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/DQ279721 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Bayes Theorem Cloning, Molecular Coatomer Protein / genetics* DNA Primers / genetics DNA, Protozoan / genetics Evolution, Molecular Genes, Protozoan Models, Genetic Molecular Sequence Data Phylogeny Protozoan Proteins / genetics* Sequence Homology, Amino Acid Species Specificity Toxoplasma / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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T 32 7030//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Coatomer Protein; 0/DNA Primers; 0/DNA, Protozoan; 0/Protozoan Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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