Document Detail

Molecular evolution of the vesicle coat component betaCOP in Toxoplasma gondii.
MedLine Citation:
PMID:  17485226     Owner:  NLM     Status:  MEDLINE    
Coatomer coated (COPI) vesicles play a pivotal role for multiple membrane trafficking steps throughout the eukaryotic cell. Our focus is on betaCOP, one of the most well known components of the COPI multi-protein complex. Amino acid differences in betaCOP may dictate functional divergence across species during the course of evolution, especially with regards to the evolutionary pressures on obligate intracellular parasites. A bioinformatic analysis of betaCOP amino acid sequences was conducted for 49 eukaryotic species. Cloning and sequence analysis of the Toxoplasma gondii betaCOP homologue revealed several amino acid insertions unique to T. gondii and one C-terminal insertion that is unique to apicomplexan parasites. These findings led us to investigate the possibility that betaCOP experienced functional divergence during the course of its evolution. Bayesian phylogenetic analysis revealed a tree consistent with pan eukaryote distribution and long-branch lengths were observed among the apicomplexans. Further analysis revealed that kinetoplast betaCOP underwent the most amount of change, leading to perhaps an overall change of function. In comparison, T. gondii exhibited subtle yet specific amino acid changes. The amino acid substitutions did not occur in the same places as other lineages, suggesting that TgbetaCOP has a role specific to the apicomplexans. Our work identifies 48 residues that are likely to be functionally important when comparing apicomplexan, kinetoplastid, and fungal betaCOP.
Sherri S Smith; Stacy L Pfluger; Elizabeth Hjort; Andrew G McArthur; Kristin M Hager
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-03-24
Journal Detail:
Title:  Molecular phylogenetics and evolution     Volume:  44     ISSN:  1055-7903     ISO Abbreviation:  Mol. Phylogenet. Evol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-22     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9304400     Medline TA:  Mol Phylogenet Evol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1284-94     Citation Subset:  IM    
Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556-0369, USA.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Bayes Theorem
Cloning, Molecular
Coatomer Protein / genetics*
DNA Primers / genetics
DNA, Protozoan / genetics
Evolution, Molecular
Genes, Protozoan
Models, Genetic
Molecular Sequence Data
Protozoan Proteins / genetics*
Sequence Homology, Amino Acid
Species Specificity
Toxoplasma / genetics*
Grant Support
T 32 7030//PHS HHS
Reg. No./Substance:
0/Coatomer Protein; 0/DNA Primers; 0/DNA, Protozoan; 0/Protozoan Proteins

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