Document Detail


Molecular evolution of functional nucleic acids with chemical modifications.
MedLine Citation:
PMID:  20714306     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nucleic acids are attractive materials for creating functional molecules that have applications as catalysts, specific binders, and molecular switches. Nucleic acids having such functions can be obtained by random screening, typically using in vitro selection methods. These methods have helped explore the potential abilities of nucleic acids and steadily contributed to their evolution, i.e., creation of RNA/DNA enzymes, aptamers, and aptazymes. Chemical modification would be a key means to further increase their performance, e.g., expansion of function diversity, enhancement of activity, and improvement of biostability for biological use. Indeed, in the past two decades, random screening involving chemical modification, post-SELEX chemical modification, and rational design methods have been advanced, and combining and integrating these methods may produce a new class of functional nucleic acids. This review focuses on the effectiveness of chemical modifications on the evolution of nucleic acids as functional molecules and the outlook for related technologies.
Authors:
Masayasu Kuwahara; Naoki Sugimoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-08-09
Journal Detail:
Title:  Molecules (Basel, Switzerland)     Volume:  15     ISSN:  1420-3049     ISO Abbreviation:  Molecules     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-17     Completed Date:  2010-12-03     Revised Date:  2010-12-15    
Medline Journal Info:
Nlm Unique ID:  100964009     Medline TA:  Molecules     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  5423-44     Citation Subset:  IM    
Affiliation:
Chemistry Laboratory of Artificial Biomolecules (CLAB), Graduate School of Engineering, Gunma University, Kiryu, Gunma, Japan. kuwahara@chem-bio.gunma-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Aptamers, Nucleotide / chemistry
Base Sequence
Directed Molecular Evolution*
Enzymes / metabolism
Molecular Sequence Data
Nucleic Acids / chemical synthesis*,  chemistry*
SELEX Aptamer Technique
Chemical
Reg. No./Substance:
0/Aptamers, Nucleotide; 0/Enzymes; 0/Nucleic Acids

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