| Molecular evolution of HA1 in influenza A (H3N2) viruses isolated in Japan from 1989 to 2006. | |
| | |
MedLine Citation:
|
PMID: 19158442 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: To evaluate the trend of phylogenetic evolution among influenza A (H3N2) viruses isolated in Gunma and A (H3N2) vaccine strains, we studied the transition of gene mutations and amino acid substitution of the sites A and B in HA1 during long-term seasons. METHODS: A total of 15 A (H3N2) strains were obtained from patients in Gunma, Japan, during the 1989-2006 seasons. A partial HA1 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The phylogenetic tree and amino acid sequences were determined with nucleotide sequences. RESULTS: We found that the nucleotide sequences of the isolates in the 1989-1990 and 1997-1998 seasons were similar to those of the A/Beijing/352/89 and the A/Sydney/5/97 strains, respectively, indicating a lag between the circulated virus and the vaccine strains. Phylogenetic analysis made possible to classify into two clusters in genetic evolutions among these strains, which showed large antigenic conversion. Moreover, we confirmed that basic amino acids tended to substitute uncharged polar amino acids, like K135T (lysine-to-threonine substitution at position 135), K145N, H155T, K156Q, R189S and R197Q, as confirmed by multiple alignment of amino acid sequences. CONCLUSIONS: We showed a new understanding of genetic evolution and amino acid substitutions of HA1 to recur pandemic. |
| | |
Authors:
|
Mika Saitoh; Hirokazu Kimura; Kunihisa Kozawa; Akira Shoji |
Publication Detail:
|
Type: Journal Article Date: 2009-01-22 |
Journal Detail:
|
Title: Intervirology Volume: 51 ISSN: 1423-0100 ISO Abbreviation: Intervirology Publication Date: 2008 |
Date Detail:
|
Created Date: 2009-04-07 Completed Date: 2009-09-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0364265 Medline TA: Intervirology Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 377-84 Citation Subset: IM |
Copyright Information:
|
Copyright 2009 S. Karger AG, Basel. |
Affiliation:
|
Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Cell Line / virology Evolution, Molecular* Genetic Variation Hemagglutinin Glycoproteins, Influenza Virus / chemistry, genetics* Humans Influenza A Virus, H3N2 Subtype / classification*, genetics*, isolation & purification Influenza, Human / genetics, immunology, virology* Japan Lectins / chemistry*, genetics Phylogeny Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Sequence Homology, Nucleic Acid |
| Chemical | |
Reg. No./Substance:
|
0/Hemagglutinin Glycoproteins, Influenza Virus; 0/Lectins; 0/hemagglutinin I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Association of cognitive impairment with serum lipid/lipoprotein among Chinese nonagenarians and cen...
Next Document: Neurovascular pulsatile compression and neurosurgical decompression of the rostral ventrolateral med...