Document Detail

Molecular evaluation of abnormalities of the short arm of chromosome 1 in neuroblastoma.
MedLine Citation:
PMID:  1980608     Owner:  NLM     Status:  MEDLINE    
Cytogenetic analyses have documented the consistent deletion of part of the short arm of chromosome 1 in neuroblastoma cells suggesting the presence of a suppressor gene in this chromosomal region. To determine, the smallest region of deletion overlap at the molecular level on independently derived tumors and to define the location of the breakpoints more precisely, Southern analyses were performed on a somatic cell hybrid panel containing the normal and altered chromosomes 1 from seven neuroblastoma lines. By this method we were able to analyze a panel of 20 cloned sequences and two isozymes to determine the location of the breakpoints. Our findings indicate that the proximal breakpoints of chromosome 1 deletions ranged over a distance of more than 50 cM with the most distal deletion breakpoint occurring between MYCL1 and D1S57. In addition, using restriction fragment length polymorphisms, it was determined that in at least three of the five cell lines in which MYCL1 was deleted from a chromosome 1, the gene was translocated to another chromosome thus retaining the diploid complement. We propose that the neuroblastoma susceptibility gene is located distal to MYCL1 and that there is another gene which is linked to MYCL1 that may be involved in this neoplasm.
J D Hunt; A Tereba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes, chromosomes & cancer     Volume:  2     ISSN:  1045-2257     ISO Abbreviation:  Genes Chromosomes Cancer     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1991-03-08     Completed Date:  1991-03-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9007329     Medline TA:  Genes Chromosomes Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  137-46     Citation Subset:  IM    
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 38101-0318.
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MeSH Terms
Child, Preschool
Chromosome Deletion*
Chromosomes, Human, Pair 1 / ultrastructure*
Gene Expression Regulation, Neoplastic
Genes, myc
Genetic Markers
Hybrid Cells
Neuroblastoma / genetics*
Polymorphism, Restriction Fragment Length
Proto-Oncogene Proteins c-myc / genetics
Translocation, Genetic*
Tumor Cells, Cultured / ultrastructure
Grant Support
CA-34759/CA/NCI NIH HHS; P30 CA-21765-10/CA/NCI NIH HHS
Reg. No./Substance:
0/Genetic Markers; 0/Proto-Oncogene Proteins c-myc

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