Document Detail

Molecular epidemiology of tuberculosis in Sicily, Italy: what has changed after a decade?
MedLine Citation:
PMID:  25407589     Owner:  NLM     Status:  Publisher    
BackgroundWe aimed to investigate the molecular epidemiology of Mycobacterium tuberculosis complex (MTBC) isolates in the province of Palermo, Sicily, Italy, by characterizing 183 isolates identified in the years 2004¿2012. A comparison with 104 MTBC strains identified in the same geographic area in the years 1994¿2000 was also carried out.MethodsOne hundred eighty-three MTBC isolates identified in Palermo, Italy, in the years 2004¿2012 were analyzed by spoligotyping and the 24 mycobacterial interspersed repetitive unit (MIRU)-variable-number tandem-repeat (VNTR) method typing. Susceptibility testing to streptomycin, isoniazid, rifampin and ethambutol was also performed. Furthermore, the spoligotyping dataset obtained from 104 MTBC isolates identified from 1994 to 2000 was reanalyzed. Distribution into lineages and clustering of isolates in the two periods was compared.ResultsOne hundred seventy-seven out of the 183 isolates of MTBC submitted to molecular typing were fully characterized. Of these, 108 were from Italian-born and 69 from foreign-born individuals. Eleven different lineages and 35 families-subfamilies were identified with the most represented lineages being Haarlem (26.5%), T (19.2%), LAM (13.6%) and S (8.5%). Except for the Haarlem lineage, where isolates from foreign-born patients were overrepresented, the distribution of isolates in the families belonging to the Euro-American clone reflected the proportions of the two subpopulations. A total of 27 (15.2%) strains were clustered and three clusters were mixed. Approximately 25% of the 183 MTBC isolates under study proved to be resistant to at least one antiTB drug, with only three isolates categorized as multidrug resistant (MDR). When MTBC isolates identified in the years 1994¿2000 were reanalyzed, lineages T (30.8%), LAM (29.8%), Haarlem (16.3%) and S (13.5%) proved to be predominant. No MTBC isolates belonging to CAM, U, CAS, Turkish and Ural lineages were identified.ConclusionsA wide heterogeneity was detected among the MTBC strains isolated in the years 2004¿2012. Six lineages were not present among the isolates of the period 1994¿2000. Comparison between distribution of lineages in the two consecutive periods depicts rapid and deep changes in the TB epidemiology in Palermo, Italy. An universal and continued laboratory-based surveillance of TB in Sicily is required.
Celestino Bonura; Michel K Gomgnimbou; Guislaine Refrégier; Aurora Aleo; Teresa Fasciana; Anna Giammanco; Christophe Sola; Caterina Mammina
Related Documents :
25342919 - Evaluation of bactericidal activity of hannon honey on slowly growing bacteria in the c...
25193039 - A first case of new delhi metallo-β-lactamase-7 in an escherichia coli st648 isolate in...
25181399 - Early dissemination of oxa-72-producing acinetobacter baumannii strain in colombia: a c...
19679019 - What's growing on your stethoscope? (and what you can do about it).
25373329 - Sialidase production and genetic diversity in clostridium perfringens type a isolated f...
2202809 - Susceptibility of oral bacteria to phenoxyethanol and phenoxyethanol/chlorhexidine comb...
1520959 - In vitro activity of antifungal agents against clinical isolates of aspergilli.
20522739 - Staphylococcus aureus nonribosomal peptide secondary metabolites regulate virulence.
2304199 - Experimental avian pmv-2 infection in a domesticated wild host: daily behavior and effe...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-19
Journal Detail:
Title:  BMC infectious diseases     Volume:  14     ISSN:  1471-2334     ISO Abbreviation:  BMC Infect. Dis.     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-11-19     Completed Date:  -     Revised Date:  2014-11-20    
Medline Journal Info:
Nlm Unique ID:  100968551     Medline TA:  BMC Infect Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  602     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Stable inverted small molecular organic solar cells using a p-doped optical spacer.
Next Document:  Are gloves sufficiently protective when hairdressers are exposed to permanent hair dyes? An in vivo ...