| Molecular epidemiology of rotavirus in Delhi. | |
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MedLine Citation:
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PMID: 15471146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rotavirus is now established as an important cause of childhood diarrhoea throughout the world. Despite the availability of more advanced techniques for HRV characterization, electropherotyping was employed in this study to demonstrate the occurrence of diverse electropherotypes & any aberrant types thereof in isolates from children with acute gastroenteritis in Delhi, keeping in mind the ease of availability, performance and discriminatory power. Faecal specimens from 1172 children suffering from acute gastroenteritis were subjected to enzyme linked immunosorbent assay (ELISA) and polyacrylamide gel electrophoresis (PAGE). A total of 158 rotavirus strains were detected and electropherotyped by PAGE. Rotavirus was detected in 13.48% of the samples. A total of 10 electropherotypic patterns were observed to be in circulation. There was predominance of the long type over the short type and long type G was the most common isolate. The present study highlights the simultaneous coexistence of different electropherotypes of Human rotavirus strains circulating in Delhi and stresses the need for constant monitoring of the genomic diversity resulting from extensive genomic variation among Rotaviruses. |
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Authors:
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Anita Chakravarti; Deepti Rawat; Arpita Chakravarti |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Indian journal of pathology & microbiology Volume: 47 ISSN: 0377-4929 ISO Abbreviation: Indian J Pathol Microbiol Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-10-08 Completed Date: 2004-11-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7605904 Medline TA: Indian J Pathol Microbiol Country: India |
Other Details:
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Languages: eng Pagination: 90-3 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Maulana Azad Medical College, New Delhi, India. dochak@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Child, Preschool Gastroenteritis / epidemiology, virology* Humans India / epidemiology Infant Infant, Newborn Molecular Epidemiology RNA, Viral / genetics, isolation & purification Rotavirus / classification, genetics Rotavirus Infections / epidemiology, virology* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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