Document Detail


Molecular epidemiology in the care of patients.
MedLine Citation:
PMID:  10539897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several different epidemiologic typing methods have been applied in studies of microbial pathogens. These methods include the more traditional nonmolecular approaches as well as the more sophisticated molecular typing methods. Application of traditional epidemiologic typing methods, such as antibiogram, serotyping, biotyping, and phage typing, have occasionally been useful in describing the epidemiology of infectious diseases. However, these methods have generally been considered to be too variable, labor intensive, and slow to be of practical value in epidemiologic investigations. In response to these limitations, several techniques have been adopted from the molecular biology field for use as epidemiologic typing methods and have been applied in studies of bacteria, fungi, viruses, and protozoa. The most widely used molecular typing methods are the DNA-based methods, such as plasmid profiling, restriction endonuclease analysis of plasmid and genomic DNA, Southern hybridization analysis using specific DNA probes, and chromosomal DNA profiling using either pulsed-field gel electrophoresis or polymerase chain reaction-based methods. The various molecular typing methods may be applied to the investigation of outbreaks of infections or may be used in the context of epidemiologic surveillance. For outbreak investigation, typing methods are used to compare isolates from a suspected outbreak to delineate clonally related and unrelated strains with the goal of short-term control of transmission. In the context of epidemiologic surveillance, molecular typing methods may be used to monitor geographic spread and prevalence shifts of epidemic and endemic clones with the goal of long-term evaluation of preventive strategies or for the detection and monitoring of emerging and reemerging infections. The specific typing method selected may vary with the task at hand; however, the typing studies must always be used to supplement, rather than replace, careful epidemiologic investigation.
Authors:
M A Pfaller
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Archives of pathology & laboratory medicine     Volume:  123     ISSN:  0003-9985     ISO Abbreviation:  Arch. Pathol. Lab. Med.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-11-30     Completed Date:  1999-11-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7607091     Medline TA:  Arch Pathol Lab Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1007-10     Citation Subset:  AIM; IM    
Affiliation:
Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Infections / epidemiology,  microbiology
Bacterial Typing Techniques
Drug Resistance, Microbial / genetics
Genetic Techniques
Genotype
Humans
Microbiological Techniques*
Molecular Epidemiology*
Phenotype

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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