Document Detail

Molecular epidemiology of classical swine fever in the Russian Federation.
MedLine Citation:
PMID:  14633204     Owner:  NLM     Status:  MEDLINE    
The ability to discriminate between various classical swine fever virus (CSFV) strains and isolates is a prerequisite for following the spread of the virus after an outbreak. To determine the relatedness between Russian CSFV isolates from different geographical regions, three fragments of the viral genome (5' NTR, the variable region of the E2 gene and a fragment of the NS5B gene) were sequenced and used for genetic typing. Thirty-one field isolates were obtained from CSF outbreaks which occurred between 1994 and 1999. In addition, three attenuated strains were included in the study, namely the LK and CS vaccine strains, and the moderately virulent 238H isolate. The vaccine strains have been used in Russia for more than 30 years. Our results showed that all field isolates are in subgroup 1.1 together with Alfort 187 and with the highly virulent strain Shimen. In contrast, the CS and LK vaccine strains belong to subgroup 1.2. While there is no evidence for the reversion of the two vaccine strains to wild type, it is feasible that the highly virulent Shimen strain, which has been used as a challenge strain for many years, contributed to field strain generation. The Russian field isolates from the 1990s can be distinguished from the CSF virus isolates which occurred in the EU Member States in the same decade, as here all outbreaks were caused by CSF viruses belonging to subgroup 2.
A Vlasova; T Grebennikova; A Zaberezhny; I Greiser-Wilke; G Floegel-Niesmann; V Kurinnov; T Aliper; E Nepoklonov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of veterinary medicine. B, Infectious diseases and veterinary public health     Volume:  50     ISSN:  0931-1793     ISO Abbreviation:  J. Vet. Med. B Infect. Dis. Vet. Public Health     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-11-24     Completed Date:  2003-12-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955260     Medline TA:  J Vet Med B Infect Dis Vet Public Health     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  363-7     Citation Subset:  IM    
NARVAC R&D Department, Ivanovski Virology Institute, Moscow, Russia.
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MeSH Terms
Base Sequence
Classical Swine Fever / epidemiology*,  prevention & control,  virology*
Classical swine fever virus / genetics*,  isolation & purification
DNA Primers
Disease Outbreaks / veterinary*
Molecular Sequence Data
RNA, Viral / analysis*
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Russia / epidemiology
Sequence Homology, Nucleic Acid
Viral Vaccines
Reg. No./Substance:
0/DNA Primers; 0/RNA, Viral; 0/Viral Vaccines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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