Document Detail


Molecular epidemiology of an SHV-5 extended-spectrum beta-lactamase in enterobacteriaceae isolated from infants in a neonatal intensive care unit.
MedLine Citation:
PMID:  8645840     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Klebsiella oxytoca that produced extended-spectrum beta-lactamase (ESBL) and were resistant to ceftazidime were isolated from infants in a neonatal intensive care unit (NICU). During a 30-week period, 3 infants developed infections and an additional 60 infants were colonized with these bacteria. The molecular typing data suggested transmission of a single strain of ceftazidime-resistant K. oxytoca among 48 of the 63 infants. The ESBL of 46 of the 48 similar isolates, 14 of the remaining 15 isolates, and 6 other Enterobacteriaceae appeared to be associated with a conjugative plasmid of approximately 85 kb. The ESBL gene was cloned, and DNA sequencing confirmed that the ESBL was an SHV-5. Hybridization data suggested that the SHV-5 gene was transmitted to other Enterobacteriaceae in vivo. The spread of the ESBL was reduced through adherence to infection control practices.
Authors:
R A Venezia; F J Scarano; K E Preston; L M Steele; T P Root; R Limberger; W Archinal; M A Kacica
Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Clinical infectious diseases : an official publication of the Infectious Diseases Society of America     Volume:  21     ISSN:  1058-4838     ISO Abbreviation:  Clin. Infect. Dis.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-07-25     Completed Date:  1996-07-25     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  9203213     Medline TA:  Clin Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  915-23     Citation Subset:  IM    
Affiliation:
Department of Pathology and Laboratory Medicine, Albany Medical Center Hospital, New York 12208, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Typing Techniques
Ceftazidime / therapeutic use
Cephalosporin Resistance / genetics
Cephalosporins / therapeutic use
Cloning, Molecular
Cross Infection / drug therapy,  epidemiology*,  microbiology
Disease Outbreaks
Fatal Outcome
Genes, Bacterial
Humans
Infant
Infant, Newborn
Intensive Care, Neonatal*
Isoelectric Focusing
Klebsiella / enzymology*,  isolation & purification
Klebsiella Infections / drug therapy,  epidemiology*,  microbiology
Plasmids
beta-Lactamases / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Cephalosporins; 78439-06-2/Ceftazidime; EC 3.5.2.6/beta-Lactamases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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