Document Detail

Molecular epidemiological study of vertical transmission of vaginal Lactobacillus species from mothers to newborn infants in Japanese, by arbitrarily primed polymerase chain reaction.
MedLine Citation:
PMID:  11957119     Owner:  NLM     Status:  MEDLINE    
We investigated mother-to-newborn infant transmission of Lactobacillus species in Japanese by the typing of isolates from the vagina of pregnant women and stool specimens from their newborn infants. All infants were born by uncomplicated vaginal delivery and were basically fed breast milk. Lactobacillus strains were fingerprinted by arbitrarily primed polymerase chain reaction (AP-PCR), using ERIC1R and ERIC2 primers. Of 86 pregnant women tested, 71 (82.6%) were positive for vaginal lactobacilli. At 5 days after birth, 24 (33.8%) of the 71 infants whose mothers were lactobacilli-positive had fecal lactobacilli, while only 1 (6.7%) of the 15 infants delivered from the vaginal lactobacilli-negative mothers was lactobacilli-positive ( P < 0.01). Lactobacillus crispatus was the most prevalent species of vaginal lactobacilli in mothers and of fecal lactobacilli in infants at 5 days of age, whereas Lactobacillus gasseri was the most common in infants at 1 month of age. Identification to the species level, followed by AP-PCR typing, demonstrated that 23.3% of the 86 infants were likely to be colonized in the intestine by the vaginal lactobacilli of their mothers, and that only 2 of the infants retained the same vaginal lactobacilli until 1 month of age. These results suggest that approximately one-fourth of infants acquire vaginal lactobacilli from their mothers at birth, and that the acquired lactobacilli do not last in the intestine of the infant long-term, but rather, are replaced by ones from milk or unknown sources after birth. AP-PCR with primers ERIC1R and ERIC2 is indicated as a potential tool for the typing of Lactobacillus strains.
Yoshiko Matsumiya; Naoki Kato; Kunitomo Watanabe; Haru Kato
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy     Volume:  8     ISSN:  1341-321X     ISO Abbreviation:  J. Infect. Chemother.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-16     Completed Date:  2002-05-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9608375     Medline TA:  J Infect Chemother     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  43-9     Citation Subset:  IM    
Institute of Anaerobic Bacteriology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500-8705, Japan.
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MeSH Terms
Bacterial Typing Techniques
Feces / microbiology
Infant, Newborn
Infectious Disease Transmission, Vertical*
Lactobacillus / classification,  genetics,  isolation & purification*
Milk, Human / microbiology
Polymerase Chain Reaction / methods*
Vagina / microbiology*

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