Document Detail

Molecular and epidemiological study of the first outbreak of vanB type vancomycin-resistant Enterococcus faecalis in Japan.
MedLine Citation:
PMID:  11326124     Owner:  NLM     Status:  MEDLINE    
In July, 1999, an outbreak of vancomycin-resistant Enterococcus faecalis (VREF) with the vanB genotype occurred for the first time in Japan at Hokushin General Hospital, Nakano City, Nagano Prefecture. Four VREF strains were isolated from the clinical specimens of four inpatients, and 16 VREF strains were isolated by the screening of asymptomatic carriers and by surveillance of the hospital environment. All of the isolates possessed vanB genes. In a pulsed-field gel electrophoresis analysis, 19 out of 20 VREF isolates exhibited the indistinguishable restriction endonuclease digestion patterns of the chromosomal DNA. Additional investigation by Southern hybridization using the vanB probe implied that the vanB gene of these 19 isolates was encoded on a 110-kb plasmid. These findings indicate that the outbreak was principally caused by a single clone. The restriction endonuclease digestion patterns of the remaining single isolate was different from those of the other isolates. The vanB gene was encoded on the chromosome.
Oana; Y Kawakami; M Ohnishi; M Ishikawa; M Hirota; M Tozuka; K Atarashi; K Baba; K Fujiki; M Okazaki; T Honda; T Hayashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of infectious diseases     Volume:  54     ISSN:  1344-6304     ISO Abbreviation:  Jpn. J. Infect. Dis.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-04-30     Completed Date:  2001-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100893704     Medline TA:  Jpn J Infect Dis     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  17-22     Citation Subset:  IM    
School of Allied Medical Sciences, Sinshu University, Matsumoto 390-8621, Japan.
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MeSH Terms
Aged, 80 and over
Bacterial Proteins / genetics*
Blotting, Southern
DNA Restriction Enzymes
Disease Outbreaks*
Drug Resistance, Microbial
Electrophoresis, Gel, Pulsed-Field
Enterococcus faecalis / drug effects*,  genetics,  isolation & purification
Gram-Positive Bacterial Infections / epidemiology*,  microbiology
Japan / epidemiology
Vancomycin Resistance*
Reg. No./Substance:
0/Bacterial Proteins; 0/VanB protein, Enterococcus; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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