Document Detail

Molecular docking of substrates and inhibitors in the catalytic site of CYP6B1, an insect cytochrome p450 monooxygenase.
MedLine Citation:
PMID:  12968075     Owner:  NLM     Status:  MEDLINE    
Furanocoumarins represent plant toxins that are used in the treatment of a variety of skin diseases and are metabolized by cytochrome p450 monooxygenases (p450s) existing in insects such as Papilio polyxenes (the black swallowtail). To elucidate the active site in the CYP6B1 protein that is the principal p450 existing in this species, we have constructed a homology model of it based on sequence and structure alignments with the bacterial CYP102 protein whose crystal structure has been defined and with the insect CYP6B4 protein that also metabolizes furanocoumarins. In the derived CYP6B1 model, Phe116 and His117 in SRS1, Phe371 in SRS5 and Phe484 in SRS6 contribute to the formation of a resonant network that stabilizes the p450's catalytic site and allows for interactions with its furanocoumarin substrates. The first two of these residues are absolutely conserved in all members of the insect CYP6B subfamily and the last two are variable in different members of the CYP6B subfamily. A combination of theoretical and experimental docking analyses of two substrates (xanthotoxin and bergapten) and two inhibitors (coumarin and pilocarpine) of this p450 provide significant information on the positioning of furanocoumarins within this catalytic pocket. Molecular replacement models based on the results of variations at two of these critical amino acids provide support for our furanocoumarin-docked model and begin to rationalize the altered substrate reactivities observed in experimental analyses.
Jerome Baudry; Weimin Li; Liping Pan; May R Berenbaum; Mary A Schuler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Protein engineering     Volume:  16     ISSN:  0269-2139     ISO Abbreviation:  Protein Eng.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-09-11     Completed Date:  2004-04-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8801484     Medline TA:  Protein Eng     Country:  England    
Other Details:
Languages:  eng     Pagination:  577-87     Citation Subset:  IM    
School of Chemical Sciences, University of Illinois at Urbana-Champaign, 190 ERML, 1201 West Gregory Drive, Urbana, IL 61801, USA.
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MeSH Terms
Amino Acid Substitution
Aryl Hydrocarbon Hydroxylases / antagonists & inhibitors,  chemistry*
Catalytic Domain
Computer Simulation*
Coumarins / chemistry
Insect Proteins / chemistry*
Methoxsalen / analogs & derivatives*,  chemistry
Models, Molecular
Pilocarpine / chemistry
Protein Binding
Psoralens / chemistry
Structural Homology, Protein
Grant Support
Reg. No./Substance:
0/Coumarins; 0/Insect Proteins; 0/Psoralens; 298-81-7/Methoxsalen; 484-20-8/5-methoxypsoralen; 91-64-5/coumarin; 92-13-7/Pilocarpine; EC Hydrocarbon Hydroxylases; EC P-450 CYP6B1 (butterfly)

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