Document Detail


Molecular dissection of the mouse zona pellucida: an electron-microscopic perspective utilizing high-resolution colloidal-gold labeling methods.
MedLine Citation:
PMID:  17072018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The zona pellucida (ZP) is an extracellular coat that encloses growing oocytes, ovulated eggs, and preimplantation embryos in mammals. The ZP contains receptors that mediate initial interactions between the sperm and egg and the relative species-specificity during gamete interaction. It also prevents polyspermy and protects the developing embryo prior to implantation. The current model of the organization of mouse ZP depicts this extracellular matrix as an extensive three-dimensional array of long interconnected filaments of a structural repeat. Using high-resolution colloidal-gold labeling methods in combination with specific lectins and monoclonal antibodies against three major ZP glycoproteins, we have characterized the outer and inner mouse ZP during folliculogenesis and shown the modifications of the ZP after ovulation and fertilization. Our immunogold labeling results also indicate the involvement of a vesicular aggregate, a specialized subcellular compartment in the oocyte, in the synthesis and secretion of ZP glycoproteins.
Authors:
Frederick W K Kan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  347     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-10-30     Completed Date:  2007-01-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  289-304     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, Queen's University, Kingston, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cricetinae
Egg Proteins / analysis,  metabolism
Female
Glycoproteins / analysis*,  metabolism
Gold Colloid / chemistry
Membrane Glycoproteins / analysis,  metabolism
Mesocricetus
Mice
Mice, Inbred Strains
Microscopy, Electron / methods*
Ovarian Follicle / physiology
Ovulation
Receptors, Cell Surface / analysis,  metabolism
Zona Pellucida / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Egg Proteins; 0/Glycoproteins; 0/Gold Colloid; 0/Membrane Glycoproteins; 0/Receptors, Cell Surface; 0/zona pellucida glycoproteins

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