| Molecular diagnosis of viral diseases, present trends and future aspects A view from the OIE Collaborating Centre for the Application of Polymerase Chain Reaction Methods for Diagnosis of Viral Diseases in Veterinary Medicine. | |
| | |
MedLine Citation:
|
PMID: 17224207 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The emergence and re-emergence of transboundary animal diseases (TADs), e.g., foot-and-mouth disease, classical swine fever and the highly pathogenic avian influenza strongly indicate the need for the development of powerful and robust new diagnostic methods. The experiences of an OIE-Collaborating Centre and of two EU project consortia are summarised on the diagnostic application of gel-based PCR, general PCR systems, phylogeny, molecular epidemiology, real-time PCR (TaqMan, Molecular Beacons, Primer-Probe Energy Transfer), amplification without thermocycling (Invader), multiplex PCR, nucleic acid extraction and pipetting robotics, automation and quality control, including internal controls. By following the steps of OIE validation, the diagnostic assays are nationally and internationally standardised. The development of padlock probes and microarrays, as well as ultra rapid PCR and sequencing methods is further improving the arsenal of nucleic acid based molecular diagnosis. Further trends of diagnostic development are also mentioned, in order to combat TADs and other viral infections more effectively in the future. |
| | |
Authors:
|
Sándor Belák |
Related Documents
:
|
19377997 - Nucleic acid pool preparation and characterization. 11537767 - Self-instructed condensation of amino acids into polymers. 2471727 - Ethidium bromide- and propidium iodide-pta staining of nucleic acids at the electron mi... 16080197 - Innate immune recognition of nucleic acids: beyond toll-like receptors. 3689367 - Butryic acid analogues augment gamma globin gene expression in neonatal erythroid proge... 9280187 - Whole-body protein kinetics in children with kwashiorkor and infection: a comparison of... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2006-12-28 |
Journal Detail:
|
Title: Vaccine Volume: 25 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 2007 Jul |
Date Detail:
|
Created Date: 2007-07-16 Completed Date: 2007-09-20 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8406899 Medline TA: Vaccine Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 5444-52 Citation Subset: IM |
Affiliation:
|
Department of Virology, The National Veterinary Institute and the Swedish University of Agricultural Sciences, Uppsala, Sweden. sandor.belak@sva.se |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Molecular Diagnostic Techniques* / instrumentation Polymerase Chain Reaction / instrumentation, methods* Veterinary Medicine / methods* Virus Diseases / diagnosis, veterinary* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Gorse GJ, et al. "Immunogenicity and tolerance of ascending doses of a recombinant protective antige...
Next Document: Challenges in the successful control of the avian coccidia.