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Molecular diagnosis of francisellosis, a systemic granulomatous inflammatory disease in Atlantic cod, Gadus morhua L.
MedLine Citation:
PMID:  21125329     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A PCR-based assay for the detection of Francisella noatunensis causing francisellosis in Atlantic cod, Gadus morhua has been developed. Seven sets of primers targeting the flanking regions of the genes (rpoA, sdhA, atpA, rpoB, pgm, groEL and 16S rRNA) of the pathogen were designed. Among the primers, groEL was found to be the most suitable gene candidate for detecting the pathogen, due to its high sensitivity at various annealing temperatures and specificity in detection. The detection limit of the assay was 100 pg of bacterial DNA per milliliter or 100 fg bacterial DNA (approximately 50 genome equivalents) per PCR reaction, however, the sensitivity of the reaction decreased by 1 log dilution in the presence of 1 mg mL(-1) of serum and mucus samples as inhibitors. Nevertheless, the assay can potentially be used as a direct and non-lethal method to detect the pathogen in fish. Thus this PCR assay is a specific and sensitive molecular method to diagnose francisellosis in Atlantic cod, and will be helpful for controlling the infection through prompt detection of the disease in farms.
Authors:
Amod Kulkarni; Christopher Marlowe A Caipang; Kjetil Korsnes; Monica F Brinchmann; Viswanath Kiron
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-03
Journal Detail:
Title:  Veterinary research communications     Volume:  35     ISSN:  1573-7446     ISO Abbreviation:  Vet. Res. Commun.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100520     Medline TA:  Vet Res Commun     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  67-77     Citation Subset:  IM    
Affiliation:
Faculty of Biosciences and Aquaculture, Bodø University College, Bodø 8049, Norway.
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