| Molecular diagnosis of cerebral toxoplasmosis: comparing markers that determine Toxoplasma gondii by PCR in peripheral blood from HIV-infected patients. | |
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MedLine Citation:
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PMID: 20963318 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As cerebral toxoplasmosis is the most common cerebral focal lesion in AIDS patients, this study evaluated three PCR markers for diagnosis, since some limitations remain present, such as low parasite levels in some clinical samples. The molecular markers were B22-B23 and Tg1-Tg2 (based on the B1 gene) and Tox4-Tox5 (non-coding fragment, repeated 200-300-fold). DNA samples from 102 AIDS patients with previously known diagnosis were analyzed. The cerebral toxoplasmosis group was constituted of DNA extracted from the blood of 66 AIDS patients, which was collected before or until the third day of the therapy for toxoplasmosis. DNA from the blood of 36 AIDS patients with other neurologic opportunistic infections was used as control group. Sensitivities of B22-B23, Tg1-Tg2, and Tox4-Tox5 markers were of 95.5%, 93.9%, and 89.3%, respectively. In the control group, the specificities were of 97.2% (B22-B23), 88.9% (Tg1-Tg2), and 91.7% (Tox4-Tox5). The association of at least two markers increased the PCR sensitivity and specificity. The concordance index between two markers varied from 83.3% to 93.1%. These data demonstrated that all markers evaluated here were highly sensitive for T. gondii determination, although B22-B23 has been shown to be the best. The association of two markers increases PCR sensitivity, but the procedure was more expensive and time-consuming. |
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Authors:
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Rafael Tonini Mesquita; José Ernesto Vidal; Vera Lucia Pereira-Chioccola |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases Volume: 14 ISSN: 1678-4391 ISO Abbreviation: Braz J Infect Dis Publication Date: 2010 Jul-Aug |
Date Detail:
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Created Date: 2010-10-21 Completed Date: 2011-03-21 Revised Date: 2011-10-03 |
Medline Journal Info:
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Nlm Unique ID: 9812937 Medline TA: Braz J Infect Dis Country: Brazil |
Other Details:
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Languages: eng Pagination: 346-50 Citation Subset: IM |
Affiliation:
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Instituto Adolfo Lutz, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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AIDS-Related Opportunistic Infections
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diagnosis*,
parasitology Case-Control Studies DNA Primers / genetics Genes, Protozoan / genetics* Genetic Markers / genetics* Humans Polymerase Chain Reaction Sensitivity and Specificity Toxoplasma / genetics* Toxoplasmosis, Cerebral / diagnosis*, parasitology |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Genetic Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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