| Molecular determinants of cell death induction following adenovirus-mediated gene transfer of wild-type p53 in prostate cancer cells. | |
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MedLine Citation:
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PMID: 11146439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adenoviral vectors expressing wild-type p53 (Ad-p53) induce apoptosis in different types of cancer cells. The therapeutic utility of Ad-p53 is now being evaluated in prostate-cancer patients. Bcl-2 is frequently expressed by prostate-cancer cells and has previously been shown to inhibit p53-mediated cell death following genotoxic stress. We studied the impact of bcl-2 on Ad-p53-induced cell death in human prostate-cancer cells. Human prostate-cancer cell lines LNCaP (p53 wt) and PC3 (p53 mut) were stably transfected with bcl-2. After p53 transduction, cell viability, apoptosis induction and modulation of specific apoptosis-regulatory proteins were assessed. LNCaP vector control and bcl-2-expressing cells underwent similar decreases in viability associated with apoptosis induction following Ad-p53 infection. Increased bcl-2 expression provided significant protection to PC3 cells transduced with Ad-p53. These findings are correlated with modulations in bax, bcl-2, bcl-x(L) and p21 protein levels. These data suggest that Ad-p53 may be useful in the treatment of some prostate cancers. |
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Authors:
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G Schumacher; E M Bruckheimer; A W Beham; T Honda; S Brisbay; J A Roth; C Logothetis; T J McDonnell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 91 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-02-02 Completed Date: 2001-02-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 159-66 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Wiley-Liss, Inc. |
Affiliation:
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Thoracic and Cardiovascular Surgery, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics* Apoptosis* Cyclin-Dependent Kinase Inhibitor p21 Cyclins / physiology Gene Therapy* Genes, p53* Humans Male Prostatic Neoplasms / pathology, therapy* Proto-Oncogene Proteins c-bcl-2 / analysis Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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P01 CA78778/CA/NCI NIH HHS; T32 CA67759-01/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Proto-Oncogene Proteins c-bcl-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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