Document Detail


Molecular determinants of MAO selectivity in a series of indolylmethylamine derivatives: biological activities, 3D-QSAR/CoMFA analysis, and computational simulation of ligand recognition.
MedLine Citation:
PMID:  10794685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A series of indolylmethylamine derivatives were assayed toward MAO-A and MAO-B inhibition. The K(i) values of these compounds are in the range from 0.8 to >10(6) nM for MAO-A or from 0.75 to 476000 nM for MAO-B. The most selective MAO-A or MAO-B inhibitors elicit a ratio of K(i) in the order of 1500 or 1000, respectively. Comparison of MAO-A and MAO-B CoMFA models showed that both the steric and electrostatic properties at the 5 position of the indole ring are determinant for MAO selectivity. Computational simulations of the complex between this part of the ligand and Phe-208 of MAO-A or Ile-199 of MAO-B, experimentally identified as responsible for substrate selectivity, allowed us to further characterize the nature of these enzyme-inhibitor interactions.
Authors:
J A Morón; M Campillo; V Perez; M Unzeta; L Pardo
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medicinal chemistry     Volume:  43     ISSN:  0022-2623     ISO Abbreviation:  J. Med. Chem.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-06-29     Completed Date:  2000-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9716531     Medline TA:  J Med Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1684-91     Citation Subset:  IM    
Affiliation:
Laboratori de Medicina Computacional, Unitat de Bioestadistica, Facultat de Medicina, and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Amines / chemical synthesis*
Animals
Computer Simulation
Indoles / chemical synthesis*,  pharmacology
Isoenzymes / chemistry,  genetics
Isoleucine / chemistry
Kinetics
Ligands
Male
Mitochondria, Liver / drug effects,  enzymology
Models, Molecular
Monoamine Oxidase / chemistry*,  genetics
Monoamine Oxidase Inhibitors / chemical synthesis*,  chemistry,  pharmacology
Phenylalanine / chemistry
Rats
Rats, Sprague-Dawley
Structure-Activity Relationship
Chemical
Reg. No./Substance:
0/Amines; 0/Indoles; 0/Isoenzymes; 0/Ligands; 0/Monoamine Oxidase Inhibitors; 63-91-2/Phenylalanine; 73-32-5/Isoleucine; EC 1.4.3.4/Monoamine Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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