Document Detail


Molecular detection of potato spindle tuber viroid in Razavi and Northern Khorasan provinces.
MedLine Citation:
PMID:  18819657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to identify the Potato Spindle Tuber Viroid (PSTVd) by Reverse Transcriptase-PCR. Among all plant diseases which are caused by viroids, PSTVd was the first viroid which is recognized by plant pathologist. Suspended potato tubers from Razavi and Northern Khorasan provinces collected and cultured. Leaves of cultured tubers were used for RNA extraction by PEG6000 Precipitation method. Subsequent RT-PCR reaction has been carried out using specific primers. A 359 bp fragment has been appeared after electrophoresis which has not appeared in healthy tuber samples. Digestion of obtained fragment with BamHI confirmed the sequence by producing two fragments of 119 and 240 bp. Sequencing has been done to certify the PSTVd. The sequencing result showed that infection in tested areas is caused by mild strain of PSTVd. In this project from 250 samples, 14 tubers were infected. This was the first report of occurrence of mild strain of PSTVd in Iran.
Authors:
Yazarlou Arezou; Behrouz Jafarpour; Mahrokh Falahati Rastegar; Ali Javadmanesh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pakistan journal of biological sciences: PJBS     Volume:  11     ISSN:  1028-8880     ISO Abbreviation:  Pak. J. Biol. Sci.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-09-29     Completed Date:  2008-10-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101247723     Medline TA:  Pak J Biol Sci     Country:  Pakistan    
Other Details:
Languages:  eng     Pagination:  1642-5     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, Agriculture Faculty, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran.
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MeSH Terms
Descriptor/Qualifier:
Agriculture
Base Sequence
DNA, Complementary / metabolism
Electrophoresis, Agar Gel
Genes, Viral
Iran
Molecular Sequence Data
Plant Diseases / genetics,  virology
Polyethylene Glycols / chemistry
Polymerase Chain Reaction
RNA / chemistry
Reverse Transcriptase Polymerase Chain Reaction
Solanum tuberosum / metabolism,  virology*
Viroids / genetics*
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Polyethylene Glycols; 63231-63-0/RNA

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