Document Detail


Molecular detection of minimal residual cancer in surgical margins of head and neck cancer patients.
MedLine Citation:
PMID:  19633367     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A great disappointment in head and neck cancer surgery is that 10-30% of head and neck squamous cell carcinoma (HNSCC) patients develop local recurrences despite histopathologically tumor-free surgical margins. These recurrences result from either minimal residual cancer (MRC) or preneoplastic lesions that remain behind after tumor resection. Distinguishing MRC from preneoplasic lesions is important to tailor postoperative radiotherapy more adequately. Here we investigated the suitability of quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) using human Ly-6D (hLy-6D) transcripts as molecular marker to detect MRC in surgical margins. Submucosal samples of deep surgical margins were collected from 18 non-cancer control patients and 67 HNSCC patients of whom eight had tumor-positive surgical margins. The samples were analyzed with hLy-6D qRT-PCR, and the data were analyzed in relation to the clinicohistological parameters. A significant difference was shown between the group of patients with histopathological tumor-positive surgical margins and the non-cancer control group (p<0.001), and the group of patients with histopathological tumor-free surgical margins (p=0.001). This study shows a novel approach for molecular analysis of deep surgical margins in head and neck cancer surgery. The preliminary data of this approach for detection of MRC in deep margins of HNSCC patients are promising.
Authors:
A Peggy Graveland; Michiel de Maaker; Boudewijn J M Braakhuis; Remco de Bree; Simone E J Eerenstein; Elisabeth Bloemena; C René Leemans; Ruud H Brakenhoff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular oncology : the official journal of the International Society for Cellular Oncology     Volume:  31     ISSN:  1875-8606     ISO Abbreviation:  Cell. Oncol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-07-27     Completed Date:  2009-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101219418     Medline TA:  Cell Oncol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  317-28     Citation Subset:  IM    
Affiliation:
Department of Otolaryngology/Head and Neck Surgery, VU University Medical Center, Amsterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Antigens, Ly / genetics*
Carcinoma, Squamous Cell / pathology*,  surgery
Female
Head and Neck Neoplasms / pathology*,  surgery
Humans
Male
Middle Aged
Neoplasm Recurrence, Local / diagnosis*,  genetics
Neoplasm, Residual / diagnosis*,  genetics
Tumor Markers, Biological / genetics
Chemical
Reg. No./Substance:
0/Antigens, Ly; 0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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