Document Detail


Molecular detection of human parechovirus type 1 in stool samples from children with diarrhea.
MedLine Citation:
PMID:  22771054     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Human parechovirus type 1 (HPeV-1) appears to be associated with gastrointestinal and respiratory infections, and occasionally with central nervous system symptoms. Since there is no accurate information about the incidence and epidemiology of HPeV-1 in Iran, this study was designed to describe the frequency of HPeV-1 in stool samples from children under 4 years of age with gastroenteritis. METHODS: RNA was isolated from stool suspensions and cDNA was prepared and amplified using a specific nested reverse transcription PCR (RT-PCR). We also compared this RT-PCR method with a cell culture procedure. RESULTS: Out of 472 samples, 112 (23.7%) were HPeV-1-positive. The incidence and prevalence of this virus was highest in children under 1 year old with diarrhea (p=0.036), and was higher in the spring and autumn than in summer and winter (p<0.001). More males than females were HPeV-1-positive (p<0.001). CONCLUSIONS: This is the first systematic study of the incidence and epidemiology of HPeV-1 among children with acute gastroenteritis in Iran. The results revealed that RT-PCR is a more practical, sensitive, and rapid technique for the detection of HPeV-1 directly from clinical samples than cell culture, which makes this a valuable method for epidemiology. Also, the rapid detection of virus can decrease both the unnecessary use of antibiotics and costs in clinical practice.
Authors:
Farideh Ghazi; Zohreh Ataei; Bahareh Dabirmanesh
Related Documents :
12341194 - Fertility trends among acceptors of sterilisation in an urban situation: a socio-demogr...
22364064 - Low-income children's reported motivators of and barriers to healthy eating behaviors: ...
22615824 - Prevalence of allergic disorders among primary school-aged children in madinah, saudi a...
22273244 - Using the pediatric asthma therapy assessment questionnaire to measure asthma control a...
17672284 - Role of the low affinity ige receptor (cd23) on the ige response against ascaris lumbri...
890364 - Primary immunisation and febrile convulsions in oxford 1972-5.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-4
Journal Detail:
Title:  International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases     Volume:  -     ISSN:  1878-3511     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610933     Medline TA:  Int J Infect Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Affiliation:
Department of Molecular Biology and Genetics, School of Medicine, Tehran University of Medical Sciences, PO Box 14155-6447, Tehran, Iran.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Resistance to diabetes-promoting lifestyle factors - What is the mechanism?
Next Document:  Shunt Reintervention and Time-Related Events After Norwood Operation: Impact of Shunt Strategy.