| Molecular detection of human parechovirus type 1 in stool samples from children with diarrhea. | |
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MedLine Citation:
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PMID: 22771054 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: Human parechovirus type 1 (HPeV-1) appears to be associated with gastrointestinal and respiratory infections, and occasionally with central nervous system symptoms. Since there is no accurate information about the incidence and epidemiology of HPeV-1 in Iran, this study was designed to describe the frequency of HPeV-1 in stool samples from children under 4 years of age with gastroenteritis. METHODS: RNA was isolated from stool suspensions and cDNA was prepared and amplified using a specific nested reverse transcription PCR (RT-PCR). We also compared this RT-PCR method with a cell culture procedure. RESULTS: Out of 472 samples, 112 (23.7%) were HPeV-1-positive. The incidence and prevalence of this virus was highest in children under 1 year old with diarrhea (p=0.036), and was higher in the spring and autumn than in summer and winter (p<0.001). More males than females were HPeV-1-positive (p<0.001). CONCLUSIONS: This is the first systematic study of the incidence and epidemiology of HPeV-1 among children with acute gastroenteritis in Iran. The results revealed that RT-PCR is a more practical, sensitive, and rapid technique for the detection of HPeV-1 directly from clinical samples than cell culture, which makes this a valuable method for epidemiology. Also, the rapid detection of virus can decrease both the unnecessary use of antibiotics and costs in clinical practice. |
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Authors:
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Farideh Ghazi; Zohreh Ataei; Bahareh Dabirmanesh |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-4 |
Journal Detail:
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Title: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases Volume: - ISSN: 1878-3511 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9610933 Medline TA: Int J Infect Dis Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ltd. |
Affiliation:
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Department of Molecular Biology and Genetics, School of Medicine, Tehran University of Medical Sciences, PO Box 14155-6447, Tehran, Iran. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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