Document Detail

Molecular detection of human papillomavirus in women with minor-grade cervical cytology abnormalities.
MedLine Citation:
PMID:  11163589     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Human papillomavirus (HPV) has been shown to be the major risk factor for the development of cervical carcinoma, the second most common cancer among women worldwide. Cervical cytology has been the main screening tool for detection of premalignant lesions in last 50 years. OBJECTIVE: The utility of a molecular assay for detection of HPV in cervical smears was evaluated. STUDY DESIGN: A total of 466 women with minor-grade cervical cytology abnormality supposed to be produced by HPV were included. Patients were classified into three groups: Patients with reactive changes, patients with cervical intraepithelial neoplasia grade 1 (CIN 1), and patients with cervical intraepithelial neoplasia grade 2 (CIN 2). In all patients, another cervical swab was obtained and tested for the HPV genome using the Digene Hybrid Capture II. This assay is able to distinguish between high-risk and low-risk HPV types. RESULTS: Based on cytology results, 44 patients showed reactive changes, 250 patients displayed CIN 1, and 172 patients displayed CIN 2. With the molecular assay, HPV was detected in 289/466 (62%) patients. The high-risk HPV type was present in 263 (56.4%) patients and the low-risk type in 26 (5,5%) patients. In 25% of patients with reactive changes, the HPV genome was detected. Corresponding rates for patients with CIN 1 and CIN 2 were 55 and 81%, respectively. CONCLUSION: Molecular detection of HPV should additionally be used to cytology in patients whose cervical smears display reactive changes, CIN 1, or CIN 2. The employed assay allows identification of patients who are at risk for development of high-grade cervical lesions.
A Vince; M Ivanisevic; V Harni; D Skalko; T Jeren
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology     Volume:  20     ISSN:  1386-6532     ISO Abbreviation:  J. Clin. Virol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815671     Medline TA:  J Clin Virol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  91-4     Citation Subset:  IM    
University Hospital for Infectious Diseases, Mirogojska 8, 10000 Zagreb, Croatia.
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MeSH Terms
Cervical Intraepithelial Neoplasia / diagnosis*,  pathology,  virology
Cytological Techniques
DNA, Neoplasm / analysis
DNA, Viral / analysis
Genome, Viral
Molecular Probe Techniques
Papillomaviridae / genetics*
Papillomavirus Infections / diagnosis*,  virology
Tumor Virus Infections / diagnosis*,  virology
Uterine Cervical Neoplasms / diagnosis*,  pathology,  virology
Vaginal Smears
Reg. No./Substance:
0/DNA, Neoplasm; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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