| Molecular detection of host cytokine expression in Helicobacter pylori infected patients via semi-quantitative RT-PCR. | |
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MedLine Citation:
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PMID: 20061762 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Helicobacter pylori (Hp) is a bacterium recognised as a main causative agent for the development of chronic active gastritis, peptic ulcer disease, gastric adenocarcinoma and primary gastric lymphoma. OBJECTIVE: Determination of the levels of IFN-gamma (pro-inflammatory) and IL-4 (anti inflammatory) cytokine expression as indicators of Th1 and Th2 immune responses in gastric cancer (GC) and non gastric cancer (Non GC) dyspeptic patients by gene specific RT-PCR. MATERIALS AND METHODS: Biopsy specimens were collected from three groups of gastric cancer (GC=18), non ulcer dyspepsia (NUD = 38) and peptic ulcer patients (PUD=20). Total RNA was extracted and complementary DNA was synthesised. PCR amplification was performed for HPRT, IFN-gamma and IL-4 cytokines and the intensity of each band was measured by densitometry and normalized against HPRT expression as a house keeping gene. RESULTS: Comparison of the results from different groups of patients indicated that IFN-gamma gene expression was similar in nonGC dyspeptic patients (NUD and PUD groups; 3.38 +/- 0.57,3.43 +/- 0.41, respectively) whereas, in GC patients, it was significantly higher than others (5.52 +/- 0.59; P < 0.0001). On the other hand, IL-4 gene expression showed no significant difference between NUD and GC patients (2.81 +/- 0.43,2.3 +/- 0.12 respectively), whereas the expression rate of this cytokine was significantly higher in PUD patients (3.7 +/- 0.1; P 0.05). Our data indicate an association between Th1 and Th2 immune responses and the development of gastric cancer and peptic ulcer disease respectively. |
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Authors:
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M Eshagh Hosseini; A Oghalaie; G Habibi; A Nahvijoo; Z M Hosseini; M Tashakoripoor; M Mohammadi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Indian journal of medical microbiology Volume: 28 ISSN: 1998-3646 ISO Abbreviation: Indian J Med Microbiol Publication Date: 2010 Jan-Mar |
Date Detail:
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Created Date: 2010-01-11 Completed Date: 2010-03-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8700903 Medline TA: Indian J Med Microbiol Country: India |
Other Details:
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Languages: eng Pagination: 40-4 Citation Subset: IM |
Affiliation:
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Biotechnology Research Center, Pasteur Institute of Iran, Tehran 13164, Iran. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Biopsy Female Gene Expression* Helicobacter Infections / immunology*, pathology Helicobacter pylori / immunology* Humans Interferon-gamma / biosynthesis*, genetics Interleukin-4 / biosynthesis*, genetics Male Middle Aged Reverse Transcriptase Polymerase Chain Reaction / methods* Stomach / pathology T-Lymphocytes / immunology* Young Adult |
| Chemical | |
Reg. No./Substance:
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207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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