Document Detail


Molecular detection of dopamine decarboxylase expression by means of reverse transcriptase and polymerase chain reaction in bone marrow and peripheral blood: utility as a tumor marker for neuroblastoma.
MedLine Citation:
PMID:  15322424     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A highly sensitive molecular method was used to evaluate the presence of dopamine decarboxylase (DDC) mRNA in the bone marrow and peripheral blood of patients with neuroblastoma (NB). DDC, like tyrosine hydroxylase (TH), is an enzyme involved in the catecholamine synthesis pathway and has recently been proposed as a specific marker of NB among pediatric malignancies. DDC transcript was detected in five of five NB cell lines, 10 of 10 NB primary tumors, 17 of 18 (94%) bone marrow samples, and 12 of 18 (66%) blood samples drawn at diagnosis in 18 patients affected by disseminated NB. In contrast, no PCR signal was found in 20 bone marrow samples obtained from patients with other malignancies or in eight of nine marrow and blood samples drawn from patients with localized NB (two stage 2 and seven stage 3). In addition, all marrow and blood samples obtained from NB patients at relapse revealed DDC mRNA. Furthermore, the percentage of DDC-positive samples was lower among the samples drawn from these patients during treatment. By comparison with conventional methods for disease evaluation, DDC transcript research can increase the sensitivity of NB cell detection in marrow and blood samples at diagnosis and during the treatment and follow-up of NB patients. These results suggest that finding DDC mRNA in NB patients could be a potential marker for minimal residual disease study.
Authors:
Fabio Bozzi; Roberto Luksch; Paola Collini; Felicita Gambirasio; Elena Barzanò; Daniela Polastri; Marta Podda; Bruno Brando; Franca Fossati-Bellani
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diagnostic molecular pathology : the American journal of surgical pathology, part B     Volume:  13     ISSN:  1052-9551     ISO Abbreviation:  Diagn. Mol. Pathol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-08-23     Completed Date:  2005-02-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204924     Medline TA:  Diagn Mol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  135-43     Citation Subset:  IM    
Affiliation:
Department of Medical Oncology, Division of Pediatrics, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy. fabio.bozzi@istitutotumori.mi.it
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD56 / metabolism
Bone Marrow / enzymology
Cell Line, Tumor
Child, Preschool
Dopa Decarboxylase / analysis*,  biosynthesis*
Flow Cytometry
Humans
Infant
Neuroblastoma / enzymology*
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction*
Tumor Markers, Biological / analysis*
Tyrosine 3-Monooxygenase / analysis,  biosynthesis
Chemical
Reg. No./Substance:
0/Antigens, CD56; 0/RNA, Messenger; 0/Tumor Markers, Biological; EC 1.14.16.2/Tyrosine 3-Monooxygenase; EC 4.1.1.-/Dopa Decarboxylase

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